Apoptosis And Restoration Of Human Villi During First Trimester Pregnancy After Exposuring Transviganal Ultrasound

Objective:To detect the expression of Fas and FasL and analysis their changes on the apoptosis of human villi during first trimester pregnancy after exposuring tansviganal ultrasound, exploring the role of time playing in restoration of human villi during first trimester. And this paper show doctors a theory that it was feasible to take transvaginal ultrasound for first trimester pregnancy.Methods:105 health normal first trimester pregnant wemen who have visited NO.2 Clinical Hospital of Tianjin Medical University from August 2009 to October 2009 for artificial abortion to terminate their pregnancies were randomly divided into three groups:the numbers of the three groups were 35. The group A had 35 wemen exposed to transabdominal ultrasound; the group B exposed to a fixed beam from transvaginal ultrasound with a 7.5MHz transducer for 10 min, their villi were abtained in 24 hour after exposure; the group C also exposed to transvaginal ultrasound for 10 min, but their villi were abtained 10 days later after exposure. Observe the time from exposuring transvaginal ultrasound and style of the pathological changes in the human embryo villi. The typical structure changes in the chorionic villi were observed by microscope. The expression of Fas and FasL proteins were analyzed by immunohistochemistry.Results:1. Clinical symptoms Two people had the phenomenon of vaginal blood in the group B, and 12 in the group C, there was significantly difference between the group C and the group B(P<0.05). However, no one in group A.2. H-E performance The typical structure changes observed by microscope were that some trophoblasts were hyperplasia in three groups, there was significantly different in the group B and C (P<0.0167).3. P-S performance There were Fas and FasL protein in three groups. And they appeared in plasmalemma and cytoplasm of trophoblast, showing yellow. The expression of Fas and FasL in the group B were significantly higher than that of the group A (P<0.0167). The expression of Fas and FasL in the group C were significantly lower than that in group B(P<0.0167); The expression of Fas and FasL in the chorionic villi were no difference between the group C and the group A (P> 0.0167).Conclusion:1. The findings suggest that the higher expression of Fas and FasL protein took place in chorionic villi that was abtained 24 hours later after exposuring transvaginal ultrasound. The expression of Fas and FasL protein were lower in the chorionic villi that were abtained 10 days later after exposure transvaginal ultrasound.2. Apoptosis of trophoblasts appeared in human chorionic villi exposuring ultrasound, but these changes in human chorionic villi were restored by itself. It means that apoptosis of human chorionic villi will be restored after exposuring transvaginal ultrasound.