| Objective:To investigate the intervening effect of Qi-Boosting Toxin-Resolving Formula (QBTRF) on the structures of cystoskeleton and the expressive activities of associated protein system in the cells of nasopharyngeal carcinoma (NPC) to explore the possible affect of such an effect on the biodynamic mechanism underlying their metastatic potentiality of NPC to provide experimental evidences to identify new targets for intervening the potential metastatic process of the tumor.Methods:Thirty two BALB/c nude mice were randomly divided into 4 groups at first, i.e.modeling group (MG),preventing group (PG) with QBTRF, treating group (TG) with QBTRF and positive controlling group (PCG) treated by methotrexate,and treated with various treating procedures. Implanted tumors were established by injecting CNE2 cells subcutaneously into the anterior part of right axil of each group of animals.Then, they were sacrificed to separate tumor samples in the same way as above for measuring the same set of parameters,drawing the curves from the weight of the nude mice, the curve from growth of the tumors and weight of the tumors,evaluating the inhibiting rate of tumor growing. To take samples from the dissected tumors for histopathological observation on the cellular adhesive structure, by light microscopy and electron microscopy respectively.As a result of this,a comparative analysis could be made on the changeable ultra-structural differences among various groups to explore the possible biodynamics-relating morphology.For an exploration on the biodynamic mechanism underlying the metastatic process of tumor cells, immunohistochemical ABC procedures were taken to determine the expressive activities of protein system in the terms of biodynamic correlation, such as Cdc42,β-tubulin, F-actin and Ezrin, among different groups with a comparative analysis on these indexes as determined on the paraffin embedded tissue samples.Results:As measured on the volumes of implanted tumors after treatment with a comparative analysis with that of MG, there were statistically significant differences in the volumes of tumors among the nude mice of PG, TG and PCG (P<0.05),with the inhibitory rates of tumor growing being 58.35%,30.03% and 52.94% for them respectively.For light microscopy, the tissue samples were embedded in paraffin to make series of sections respectively, stained by conventional methods of hematoxylin-eosin procedure, for comparative observation on the histopathological changes among different groups.Electron microscopy shows the medication group tumor cells a model group of filamentous skeleton fiber have some form of destruction, supplementing party prevention group of cytoskeleton sparse, skeleton fiber short and to the chronology, arrange for endless,rare stress fiber bundles.The samples for transmission electron microscopy were showed that the formation of fibrous skeletons were distroyed in an inhibited way in varying degrees.As determined by SABC immunohistochemistry followed by image analysis on the cellular adhesion-relating proteins in sections of various groups,the expressive activities of Cdc42,β-tubulin, F-actin and Ezrin were much higher in the samples of MG than that of PG, TG and PCG, with significantly statistical differences among them (P<0.01).Conclusions:It can be concluded from this experimental study that QBTRF bring about inhibitory effect on the metastatic potentiality of NPC cells via the mechanisms of expression inhibition of Cdc42 to induce decreased expressive activity of cytoskeletal filament protein F-actin and protein Ezrin microtubule proteinβ-tubulin in these cells so as to distroy the formation of fibrous skeletons in an inhibited way and bring about through its intervening effect on their biodynamic mechanism. |
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