Establishment And Experiment Of Rabbit Lung Ischemia-reperfusion Model In Pulmonary Surgery

Following with the surgical technique's rapid development, more and more patients who suffered from local advanced non-small-cell lung cancer received operations, which achieved satisfactory short-term and long-term effect. The blocking of pulmonary vessels, including the blocking of pulmonary artery and pulmonary circulation, is always applied in the surgical treatment of locally advanced non-small cell lung cancer, which could reduce bleeding and make the operation easier. This technique would provide opportunity to those who could not tolerate pneumonectomy because of their poor cardiopulmonary function. However, the blocking of pulmonary vessels will induce lung ischemia-reperfusion injury (LIRI). Is there any difference between the LIRI induced by the blocking of pulmonary artery and pulmonary circulation? And is it reversible with the LIRI in the pulmonary surgery? Now there was few systematical study on pathophysiological characteristic of lung ischemia-reperfusion injury induced by blocking pulmonary vessels. The present study was performed to establish the pulmonary vascula blocking model in rabbit and to investigate the characteristic of lung ischemia-reperfusion injury in pulmonary surgery. Objective: To establish lateral pumonary vessels blocking model in rabbit and to investigate the characteristic of lung ischemia-reperfusion injury in pulmonary surgery.Methods: 54 New Zealand rabbits were randomly divided into 3 groups: group I, control group; group II, blocking pulmonary artery; group III, blocking pulmonary artery and veins. At the time of opening chest ,1hour ischemia and 2hours reperfusion ,the changes of partial pressure of oxygen in arterial blood (PaO2 ) and the content of MDA in left lung tissue were observed. Then the water content of left lung was recorded.Results: All the rabbits survived during the observation period. There were significant differences in PaO2 , MDA and wet/dry ratio of the lung tissue between group I and the other two groups ( P<0.05 and P<0.01),and there was no significant difference between groupⅡand group III (P>0.05).Conclusion: This provides a simple, reliable model for investigating lung ischemia-reperfusion injury in pulmonary surgery. Objective To investigate the lung ischemia-reperfusion injury in pulmonary surgery.Methods 126 New Zealand rabbits were randomly divided into 3 groups: group I, control group; group II, blocking pulmonary artery; group III, blocking pulmonary artery and veins. At the time of opening chest , 1h ischemia and 1,2,4,6,24h reperfusion ,the changes of partial pressure of oxygen in arterial blood (PaO2 ) and the content of MDA in left lung tissue were observed. The water content of left lung tissue and pathological study were also recorded.Results All the rabbits survived during the observation period. There were significant differences in PaO2 , MDA and wet/dry ratio of the lung tissue between I and other two groups at the time of 1h ischemia and 1,2,4,6h reperfusion ( P<0.05 and P<0.01) ,but there was no significant difference between groupⅡand group III (P>0.05).And there was no significant difference among the three groups after 24h reperfusion. Pathological study revealed that similar injury changes happened between groupⅡand group III, and the obvious injury happened at the time of 4 hours reperfusion. At the time of 6 hours reperfusion pathological changes in both groupⅡand group III began to recover, and completely recover after 24 hours reperfusion.Conclusion The lung ischemic-reperfusion injury caused by blocking pulmonary artery is similar with that caused by blocking pulmonary artery and veins. It is safe to previously block pulmonary vessels within 1 hour during pulmonary surgery.