Raccoon Growing Season Low Protein Diet And Amino Acid Requirements Of Research

This thesis contained two parts. The first part observed the effects of diets supplemented with sulfur-containing amino acids and lysine on growing raccoon dogs to obtain appropriate level of amino acids added in diets. The second part included the study of protein requirement in growing furring period and the research of low protein diets supplemented with methionine and lysine to explor the feasibility of low protein diets in raccoon dog's feeding and appropriate concentration of amino acids in low protein diets.ExperimentⅠ: The requirement of sulfur-containing amino acids and lysine of growing raccoon dogs. 3 (three sulfur-containing amino acid levels 0.76%, 0.91%, 1.06%)×3 (three lysine levels 1.23%, 1.42%, 1.69%), two- factor experimental design. One hundred and eight male raccoon dogs at 9 weeks of age with similar body weight and good physical status were randomly allotted to nine treatment groups, and each group had 12 raccoon dogs. The test studied effects of different diets with sulfur-containing amino acids and lysine on growth performance,some biochemical parameters and serum free acids,digestion and metabolism of nutrents.The results were as follows:1 Low-protein diet supplemented with appropriate concentration of methionine and lysine could significantly improved the growth performance of growing raccoon dogs.2 Appropriate concentrations of sulfur-containing amino acids and lysine could significantly improved digestion and metabolism of nutritional matters.3 In growing period of raccoon dogs, through the growth performance,serum biochemical parameters, serum free amino acids, digestion and metabolism trials and other indicators, we concluded that the best group was group 9 with the diet supplemented with methionine and lysine ( dietary sulfur-containing amino acids 0.98% and lysine 1.54% ). The optimal concentration of methionine added in diets was 0.30% (dietary sulfur-containing amino acids 0.98%). The optimal level of lysine added in diets was 0.40% (dietary sulfur-containing amino acids 1.54%)ExperimentⅡ: Effects of protein and amino acids content on growth performance and fur parameters of the growing-furring raccoon dogs.Test 1: Single-factor experimental design. Animals were allotted randomly to three groups: P28.18 (P=Protein content %), P24.15, P22.0 and each group had fifteen animals.Test 2: Amino acids were added in base diet which was diet 3 of test 1. 3×2 two -factor experiment design: three sulfur-containing amino acid levels (S1:0.77%, S2:1.02%, S3:1.27%), two lysine levels (L1:0.81%, L2:1.11%).The experimental diets consisted of extruded corn, soybean meal, fish meal, soybean oil and so on. The diets of group 1 to 3 weren't supplemented with additional synthetic amino acid, while the diets of group 4 to 8 were supplemented with amino acid in the base diet. The test studied effects of different diets on growth performance, biochemical parameters, serum free amino acids, digestion and metabolism trials and fur quality.The results were as follows:1 The high level of dietary protein could get better performance, whereas lead to negative environmental effects and higher feed cost. To some extent, low-protein diet affected the quality of fur. Through comprehensive analysis, the diet with CP24.15 not only didn't affect growth performance and fur quality but also could achieve higher economic efficiency, so, which was the optimal diet.2 The raccoon dogs fed with low-protein diet (CP22.03) supplemented with appropriate ratio of methionine and lysine significantly increased production performance, and had no significant differences with high protein-groups with CP24.15 or CP28.18. The raccoon dogs fed diet containing sulfur-amino acids 1.27% and lysine 0.81% got the best growth performance and fur quality3 Methionine was important for fur growth. Fur quality decreased with a decline of methionine. Appropriate level of sulfur-amino acids was 1.27%.4 Diets with different lysine levels had no significant difference on growth performance and fur quality of growth-furring raccoon dogs.This paper studied the effects of dietary protein and amino acid levels on growth performance, blood biochemical parameters, serum free amino acids, digestion and metabolism and so on. The results showed that different levels of protein and amino acid had effects on the raccoon dog and the requirement of amino acid wasxc different in different periods. The application of the results would be good for raccoon dog farming and help achieve significant economic and social benefits.