Preparation And Preliminary Applications Of The Rabies Virus Colloidal Gold Test Strip

Rabies is a zoonosis by rabies virus infected,Once people are infected,100%will be dead,rabies is acute infectious disease and the highest case fatality up to now,about 50, 000 people are dead because infected rabies virus in the world every year,it is a serious problem of public health in developing country.The main reason of infection is animal(canine,cat ect.)direct or indirect touch people,mostly canine infected rabies have spread virus before clinical symptom,Appearing clinical symptom and after clinical symptom.So it is very important to diagnose RV and study on epidemiologic surveyColloidal gold immunochromatographic rapid detecting technology is more and more attention in clinical diagnosis because it's advantage,fast,sensitive and specific.The objective of the experiment is establishment colloidal gold immunochromatographic rapid detecting technology for rabies virus.The principle of the experiment is double antibody sandwich method,use McAb conjugate with gold particle,and coating lest lin with PAb.with sheep anti-mouse coating control line.The purified rabies virus ERA srain was used asantigento immunize Balb/C mice and two monoclonal antibodies(McAbs),designated MRV201,MRV301.Both are anti rabies virus nucleoprotein.With saturated ammonium sulfate and Protein-G purity,maximum concentration is 2.36mg/mL by ultraviolet spectrophotometer assay,we prepared a specific antiserum by immunizing rabbits with the purified rabies virus,neutralization antibody potency is 1:128,it is purity through Glauber's salt and Sephadex G-200 purify.and it has no other hybridprotein;goat anti-mouse IgG concentration is 0.8mg/mL.This research do some optimum combinations about influencing factor in test paper.use the completed test paper detect rabies virue positive suckling mouse brain and rabies virus cell clture fluid,the result is coincidence contrast with FAT,it will be a good found for clinical diagnosis rabies virus in the future.