Asparagus Anther Microstructure Observation And Anther Culture

In this experiment the material is the flower bud of Asparagus officinalis L. and the method is paraffin observed the dissected struction of anther of Asparagus officinalis L. Compared and analysed the flower bud of morphological character and the relation with paraffin method. From the results of anther culture of induction medium and differentiation medium and the conditions of culture found the medium which fit to grow up. Meanwhile, the sex of regeneration plant and hemerophyte was distinguished with peroxide enzyme electrophoresis. The results show:1. Transverse section of Asparagus officinalis L. is like butterfly, and consists of the medicine separate and pollen pouch. Pollen pouch involves pollen and pouch wall. Pouch wall can store the matter at first. When the anther ripe, cell expland with the stored matter disappearing. Secondary of fiber layer thicken.2. The relations were obtained between the morphological character of flower bud and cytology characteristic in different growth period.3. The most fittest hormone combination is 1.0 mg/L 6-BA and 2.0 mg/LNAA. The introduction ratio is 56.0%. Differentiation medium is the basic medium attaching with 6-BA1.0 mg/L,NAA0.2 mg/L. The most high differentiation ratio is 60%.4. Sucrose is the best origin of activated charcoal, the suitable density is 3%. Anther culture, it is advantageous for the differentiation ratio induction and the differentiation adding activated charcoal to the medium. The suitable density is 0.5%.5. The anther medium ,the best method of growing root is that choosing 0.3cm caulis point inoculate into the medium with 6-BA,NAA,IBA,KT. The best one is MS+6-BA 0.05 mg/L +NAA 0.04 mg/L+IBA1 mg/L+KT 0.4mg/L.6. The result of electrophoresis of peroxidase isoenzyme showed that male is less a enzyme belt than female. Compare to the enzyme belt of hemerophyte: we can identified the sex with regeneration plant of Asparagus officinalis L.