Endophytic Bacteria Sources With The Screening Of Anti-tumor And Antibacterial Efficiency Of Active Substances

Endophyte is a novel source for bioactive products screening. 5260 samples produced by 660 endophytes reserved by Shanghai Health Creation Center for Biopharmaceutics R&D were tested on several models, just as Cancer Model A549, LoVo and antibiotic synergist model. And the result of second round indepth-screening for all bioactive samples demonstrate that nine samples still have inhibitory ability against both cancer cells, including six ester-phase samples: HCCB1546, HCCB1778, HCCB1927, HCCB1791, HCCB1907, HCCB1540, and three aqueous-phase samples: HCCB1601, HCCB1622, HCCB1664.There is one aqueous-phase sample HCCB1072 which has the ability as the antibiotic synergist.HCCB1622 was selected from the nine bioactive strains for its strong inhibition on cancer models. This strain was identified as Trichosporium Fri. and more researches on the physiochemistry of its fermentation have done. In order to get the bioactive compound in the second metabolite, three compounds A, A2 and B were isolated by the means of solvent extraction, silica gel chromatography, TLC, and HPLC et al. Compound A and B were detected by Mass Spectrum (MS), ¹³C-NMR, ¹H-NMR-Compound A was analyzed to be one of the sterols, and compound B have the same mother nuclide like A, but more complicated, it was not found from some references related...