Cherry Rootstock Colt Antimicrobial Peptide Gene Conversion

The　shoot　apex　of　Cherry　rootstock　Colt(Prunsavium×Pruns　pseudocerasus)　was　used　as　explant.Transformation　by　Agrobacterium　tumefaciens　with　antibacterial　peptide　gene　was　studied　in　this　paper.In　case　of　the　capacity　of　transformation　to　tabaco,we　put　pSMC　to　A208SE.9　transformed　clcones　were　obtained　by　pSMC/A208SE.　The　results　of　molecular　biological　assays　with　PCR　confirmed　that　the　antibacterial　peptide　genes　had　been　integrated　into　the　genome　of　cherry.Antibacterial-resistant　test　and　inculating　with　C58　for　the　transgenic　plants　showed　that　transgenes　could　be　expressed.　High　transformation　frequency　could　be　received　when　Agrobacterium　were　re-suspended　in　activation　medium　YEB-1　(pH5.6)　with　concentration　of　OD600　　0.4.　It　was　important　to　pre-culture　explants　before　infection,　and　1　day　was　the　best.　The　shoot　apex　from　25　day-old　tube-plants　were　benefit　for　obtaining　transgenic　plants.　5～7　min　for　infection　time,　3　days　for　co-cultivation　period　,3～7　days　delay　selection　and　temperature　of　co-cultivation　with　24～26℃　were　the　best　transformation　condition.　The　highest　transformation　frequency　was　3.01±0.2%.