Mutation Breeding Of Marine Microalgae And Its Fatty Acid Determination

By treating two kinds of the ocean microalgae with EMS (shorted from Ethyl Methanesulphonate) and 60Co-γ ray radiation, we revealed the effect of the two mutagenesis on the rate constant of the cell population of Chlorella and Isochrysis galbana,and the effect of the two mutagenesis on the EPA(shorted from eicosapentaenoic acid) and DHA(shorted from docosahexaenoic acid) contents in the two ocean microalgae cells.The experimentation provided the theory of cultivating a new ocean mocroalgae variety rich in EPA and DHA and increasing rapidly in cell number.The results demonstrated that both Ethyl Methanesulphonate and 60Co-γ ray radiation have an adverse effect on the increase in the cell number of Chlorella and Isochrysis galbana,especially with 60Co-γ ray radiation,but the two mutagenesis can also increase the EPA and DHA contents in the two ocean microalgae cells,especially with EMS.We increased the EPA and DHA contents in Chlorella cell by 44.69% and 72.61% with EMS,respectively.At the same time the EPA and DHA cotents in Isochrysis galbana cell are 1.8 times and 1.4 times as much as the initial cell which wasn't treated with EMS,respectively.What s more,some saturated fatty acids contents were also increased in Chlorella and Isochrysis galbana cells in a certain exent.The effect of the two mutagenesis to the rate constant of the two microalage cell and the EPA and DHA cotents in the two miroalage cells proved that EMS was the better mutagen.