Mango Anthrax Genetic Basis Of Polymorphism

In this paper,a primary study was carried on the fungicide resistance and genetic diversity of 70 mango anthracnose isolates from 14 regions include Hainan,Guangdong,Guangxi,Sichuan et al.The genetic polymorphism of mango anthracnose (Colletotrichum gloesporioides) of 70 isolates were studied by RAPD with 19 arbitrary 10-bp primers and were clustered on coefficient.The 70 isolates were clustered into 2 groups( i.e. Group I and Group ) and 6 small groups(i.e. Group I a,Group I b, Group I c,Group I d and Group II a,Group II b ) base on their genetic similarities. The similarity coefficients are 0.306 ~ 0.928 among 6 small groups. The isolates from different regions are clustered on low coefficients and the ones from the same regions are clustered on high coefficients. It means although there is no strict correlation between the Colletotrichum gloesporioides's genetic basis and it's geographic resources,yet in the same region the genetic background of the Colletotrichum gloesporioides is similar. Fungicide resistance was tested with Colletotrichum gloesporioides growth rate and Carbendazin concentration gradience. Five gradient concentration of Carbendazin plates(i.eg/ml,5g/ml,10g/ml,25g/ml,50g/ml)were prepared and all isolates were tested with those plates. Results show some isolates have got fungicide resistant capacity. Consolidate RAPD analysis, the fungicide resistant isolates are clusterd on the low coefficence with those fungicide sensitive ones. So the genetic changing is one of the reasons for the fungicide resistance.