| In this study, we selected three crossbred generations of RRIM600 X PR 107, Reyan217 X Reyan88-13 and Reyan88-13 X IAN873, and nine high yield breeds from different parents as experiment material on the basis of their comparative dry latex yields. BSA and RAPD were used to analysis the linkage markers of dry latex yield related genes in rubber tree. The results are as follow:(1) Totally, 1000 RAPD primers were used. Altogether, we got 12 primers amplifying bands with obvious polymorphism between high dry latex yield and low dry latex yield gene pools. Among them, 5 (S309 S8 L4 J5 and C7) were from the crossbred F1 gene pools of RRIM600 X PR107 , 3 (AG19 A18 and J9) from Reyan217 Reyan88-13 and 4 (AI17 AU18 Q8 and S19) from Reyan88-13 IAN873.(2) The dry latex yield trait was shown to be quantitative trait, which is controlled by multiple genes. The dry latex yield related genes were different in the offspring from different parents. No yield related markers were found in our experiments to separate all the FI generations from the three crossbred parents. That's to say, no primer can separate all the high and low yield materials in one time amplification.(3) Primers S8 S309 were linked with dry latex yield related genes in F1 generations from crossbred RRIM PR107, and in the F1 generations that came from Reyan217 IAN873 and Reyan217 X Reyan88-13, AI17 and AG19 were related with dry latex yield respectively.(4) A marker band amplified by primer S8 was cloned and sequenced. The total sequence was recalled in Gen Bank using blast x method, and the results shown that this sequence was homologous with Brassica napus cytochrome c oxidase.(5) The RAPD linked marker obtained from primer S8 was transferred into SCAR marker successfully. |
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