Of Verticillium Dahliae (of Verticillium Dahliae) Pathogenic Form Of Aflp Analysis And Molecular Identification Of Research

In greenhouse the pathotype of 41 strains of Vertwilltum dahliae of cotton were studied by maculating the seedlings of 4 different cotton cultivars, EjingNo. 1(S)~ Zhong-12(T) WcnS(R) and TangmianNo.2(R). 41 stains were clustered into 2 groups: defoliating types and nondefoliating types. Amplified fragment length polymorphism (AFLP) was performed on 41 strains of V dahiiae of cotton in search of DNA probes specific for distinguishing between nondefoliating strains and defoliating strains. Specific bands of 433 base pairs(bp) and 1 lObp amplified only from nondefoliating strains were obtained using two pairs , E64 (GACTGCGTACCAATT CGAC) M53 (GATGAGTCCTGAGTAACCG) and E49 (GACTGCGTACCAATTCCAG) M65 (GATGAGTCCTGAGTAAGAG) ,which named EM433 and EM110, respectively. The specific band EM433 was recovered., cloned and sequenced, and 2 primers of Sequence characterized amplified regions(SCAR) markers, 5 - -CGAGACGTCCGAGTC GATATAGC-3 - and 5 - -GTCGTCCTTCTAGTGGGTATCCTCC-3 -, were deviced and systhesised in terms of the result of sequence. 41 strains of V dahliae were amplified specifically, 9 nondefoliating strains and 2 defoliating strains could yield EM.~33, and accuracy percentage rate identified come to upwards of 90%. Accordingly, specific band EM433 could be used for identifying nondefoliating strains. System cluster analysis based on 169 polymorphic bands from results amplified with 8 effective primers to generate adendrogram.4 1 strains of V. dahliae were clustered into 2 groups: 10 nondefoliating strains and 1 intermediate strain; 30 defoliating strams,including 4 control defoliating strains and 26 defoliating strains from northern cotton area. According to dendrogram, We suggest that certain relevant relationship exist in strains of different geographical source.