Boer Goat Sperm In Vitro Capacitation And Be Able To Before And After The Ultrastructural Changes

IN VITRO CAPACITAT ION OF BOER SPERM AND ITS ULTRU?STRACTURE BEFORE OR AFTER CAPACITAT ION Abstract : The objectives of this study were to select the excellent culture media lii vitro capacitafion and observe the fine structure of Boer sperm before or after capacitatiom Three cultwe media used are 5, D and B. Separate the motile spennatozoa before capacitation, and lhen exposure capacitated sperm to lysophosphatidyl-choline(LC) to induce acmsome reaction. Capacitation was evaluated by determining the number of motile aciosome-reacted sperm using TG staining. Sperm were incubated 4h by S media of0 g/ml 5p.g/mU. 10 jig/mb 15 jig/mi-. 2Ojig/mlheparin,andheparinwasaddedat 0h 1W. 2W. 3W. 3.75hafterthe start of incubation. Atthe end of incubation, Oor3Op.gIml LC was added, and the percentage of sperm acrosome-reacted was determined by TG staining after a thither 1 5mm incubation. Obtain the sperm capacitated or non-capacitated and process according to routine procedures for TEM. Results indicated S media was the best culture media allowing for its effects on capacitation, motility and viability of sperm. The percentage of acrosome-reacted sperm at 4h was significantly different from other t media (P < 0.01) . Supplements of 1 5jig-l or 20 jig/mI heparin had apparent effect on acrosome reaction of sperm (P < 0.05) . But there was no apparent difference between these two treatments (P >0.05). Induce capacitated sperm with LC for a further 15mm and obtain the results of acmsome-reacted sperm which was significantly different from any other treatments (P < 0.01) . But there were difference among individuals. The ultra-stmcture of Boer spermatozoa was similar to that of other mammals. Eveiy sections of sperm had its ox characteristics. The great morphological changes had happened after capacitation. and that maybe have significant importance in fertilization.