Dot-immunogold Silver Staining (dot-igss) Detection Of Rabbit Viral Hyperlipidemia Igg Antibodies,

A Dot-immune-gold-silver staining (Dot-IGSS) assay was developed for the detection of IgG antibody against rabbit hemorrhagic disease .The optimum working concentration of mouse anti-rabbit IgG was determined to be 1:200 that of goat anti-mouse IgG labeled colloidal gold to be 1:20 and the optimum time of silver staining to be 15 minutes. The same antigen quantity was used by Dot-lOSS, Dot-ELISA and 1-11 to detect the antibody concentration, the result showed that the average antibody concentration was 1:1436, 1:1185 and 1:14 respectively by 3 methods Dot-IGSS is 1.2 times as sensitive as Dot-ELISA, and 103 times as sensitive as HI. The specific blocking test and the cross-reaction test with rabbit clostridium welchii, rabbit pasteurella and canine parvovirus serums showed the high specificity of the Dot-lOSS. The positive rates of the 18 experimentally infected positive serum measured by the Dot-IGSS, Dot-ELISA and HI tests were respectively lOO%(18/18), 100%(18/18) and 33.3%(6/18), while the positive rates of the 96 collected samples were respectively 9.4%(9/96),7.3%(7196) and 3.10/o(3/96).The agreement rate of detection by the Dot-IGSS and Dot-ELISA was 92.4% in all the specimens. The studies indicate that the Dot-IGSS is a low-cost, facile, rapid, sensitive, specific and reproducible method for detection of anti-rabbit hemorrhagic disease serum. It is an efficient method for rapid diagnosis and epid~iological investigation of rabbit hemorrhagic disease. This studies will lay a foundation for application of Dot-IGSS in diagnose and studies of other diseases in the future.