Cloning Of Lfy Homolog Genes And Physiological Changes Of Floral Bud Differentiation From Tung Oil Tree

The Tung Oil Tree is the peculiar non-woods in our country.It has thousand years the above cultivation history at least and didn't spreaded abroad u-ntil 1880.Its unique biological character made that it had a potential ablity to become the type non-woods. This research used duinian which had early flowerage for materials, and then isolated and cloned the sequence of LFY gene.The work is the breakthough points of the floral mechanism of expressing and adjusting or controling for Tung Oil Tree.At the same time, studying the nutrition foundation and dissection features laid a physiological and biochemical grounds. The research didn't only lay a foundation for Tung Oil Tree, also provide the parties concerned research of other non-woods with convenience and offered a new way of thinking. The main result of research as follows.1 LFY part genome sequences has isolated and cloned from Tung Oil Tree leaves, It will be used biological sequence analysis software Vector NTI stitching, There were 1037 bp long. And I submitted to the NCBI website to Blast analysis, similarity of LFY genes reaching more than 88 percent to other plants, which was confirmed by the clips LFY Tung oil tree part of homologous gene fragment. Here is a fragment of 638 bp of the intron, there are two sides of 273 bp and 126 bp extron, encoding 133 amino acids, Its splicing sites was the same as other plants.2 To design primers from the genome Tung LFY and other plants. Using RT-PCR and RACE technology, combined with TD-PCR amplification to clone a section of size 643 bp sequence fragments, it was encoding 148 amino acids, contains TAA termina tion codon and Poly A tail, there are some 3'untranslated region.3 Mensurating three physiological indicators during the flower bud differentiation per-iods including dissolvable sugar, dissolvable starch, dissolvable protein. In the early periods total dissolvable sugar content in inflorescence has a clear decline than later. Then it sustained growth at stamen and pistil stage, content to the highest point. The leaves starch content changes was the same as the total sugar, with the advance of floral bud differentiation, in petals stage it is the maximum content. Protein content in the entire process of growth was the first decline after rising trend, particularly the early calyx increased rapidly during differentiation, and achieved maximum value at the calyx stage.4. Paraffin sections was using to determine the floral bud differentiation specific time in the areas of Hunan, and than studied the differentiation features.