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Hypothermia Produced Floc Bacteria To Remove The Pilot Study Of The Iron Ions

Posted on:2011-08-10Degree:MasterType:Thesis
Country:ChinaCandidate:Z Q LiuFull Text:PDF
GTID:2192360305974055Subject:Microbiology
Abstract/Summary:PDF Full Text Request
In China the groundwater resources contains abundant of iron, which needs to be removed in order to improve the utilization of ground water.In this experiment, four bacteria strains of low-temperature flocculant-producing (strain H8, H13, DN1, and DG2).which were screened from activated sludge and low temperature bioreactor were investigated in removing several metal ions in water. The study found that the iron ions could be removed effectively by four bactria strains. After the further rematch these four strains, obtained the compound flucculanting bacteria, which is constituted by H8, H13, DN1, and DG2. By using the compound flocculant-producing bacteria, the removal rate of iron in the water was over 75%.In order to improve the iron removal efficincy, the culturing conditions were optimized, including culturing temperature, pH, culturing time, and vaccinating amount.. The results showed that the best culturing condition was:after vaccinated 2% of compound flocculant-producing bacterium into the culture solution which is pH8.0, and cultivated 72h at 20℃, rotating 100r·min-1. The effective of removing iron is best by using the compound flocculant-producing bacterias cultrued under the best conditions; the removal rate of iron was over 80%.Investigating the iron removing conditions by static test, the results showed the best removing effect was:vaccinated 4% of compound flocculant-producing bacterium into the water, the water contained 10mg·L-1 iron which pH is from 6.5 to 7.0. After 5h aeration, DO3.0 mg·L-1, the removal rate of iron was over 80%.In order to avoid the bacteria running off, compound flocculant-producing bacteria were needed to be immobilized. The condition of immobilization was studied and was determined that the concentration of sodium alginate embedding medium was 4%, the proportion of the low temperature compound flocculant-producing bacterium and embedding medium was 1:2, dropping the mixed liquor into CaCl2 linker which concentration was 1%, cross linking 18 hours. The gel glocule of compound flucculanting-producing bacteria was obtained which was able to apply to iron removal.The immobilized globule of compound flocculant-producing bacteria was applied into the deferrization test facilities designed for iron removal treatment. Running parameter of the treatment was optimized and the results indicated that by putting 5% fossilize globule of compound flocculant-producing bacterium into the water, pH was 6.5-7.0, DO3.0mg·L-1, the removal rate of iron was over 80%.After 30 days running, the removal rate of iron reached to 84% by using the compound flocculant-producing bacteria. The iron removal effect was proved high effiency by compound flocculant-producing bacteria after immobilization and the treament effect could last for long time. The results above can settle the theory and practice foundation in deferrization technology project.
Keywords/Search Tags:compound flocculant-producing bacteria, microbial immobilization, biological removal of iron
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