Immunosorbent Assay To The Study Of The Magnetic Coating Of Carbon Fiber

In recent years, immunoadsorption therapy has become an important Method to Removal of pathogenic substances in blood. In this project, magnetic microspheres were fixed on the surface of the good biocompatibility carbon fibers to prepare immunoadsorption magnetic coated carbon fiber carrier.The main conclusions are as follows:(1) In process of preparation organic sol, the product is high purity nano-Fe3O4 and the outer layers of Fe3O4 particles are coated with oleic acid. The best preparation route is dropping the surface modifier during the synthesis reaction. The Fe3O4 particles can be fully coated by oleic acid to maintain good stability and dispersion when the oleic acid dosage is 0.7ml. In process of preparation Hydrosol, the products using different surfactants are high purity Fe3O4, and the modified surface of Fe3O4 particles are coated with a corresponding surface modifier. The dispersion and stability of the Fe3O4 hydrosol prepared by PEG as surface modifier was the best.(2) The magnetic coated carbon fiber prepared by Fe3O4 organic sol is composed of carbon and Fe3O4, and has smooth surface and uniform coating. In various Fe3O4 hydrosols, the Fe3O4 microspheres prepared by PEG as a surface modifier have the best coating on the surface of carbon fiber.(3) The static parallel adsorption test shows that the adsorption on the BSA of activated carbon fibers with magnetic surface treatment and activating-coupling treatment is much higher than the blank activated carbon fiber and the adsorption property is relative stability. The adsorption of immunoadsorption magnetic coated carbon fiber prepared by Fe3O4 hydrosol that PEG as surface modifier is relatively higher than prepared by Fe3O4 organic sol preparation. By orthogonal test, there are obtained the important order of the factors on the adsorption property in activation-coupling process, and gotten the best level combination.(4) In the simulation experiment of immunosorbent column, two kinds of packing structure were eliminated because of unsatisfactory, thus an alternative design was proposed, which is knitting immunoadsorption magnetic coated carbon fibers into filter cloth.