Detection Of Microcystin In Water And Aquatic Research

Microcystins are common hepatotoxic cyclic toxins released from blue and green algae and they may have hazards on animal and human health. We develop LC-MSMS methods to determinate seven microcystins (LR, RR, YR, LW, LA, LF, LY) in water, aquatic products, spires. The conditions of sample preparation, SPE, LC, mass, are optimizedWe develop two sample preparation methods for water sample such as drinking water, environmental water. One is direct sample method. LOQ is 0.5μg/L, the recoveries are between 82%-110%, RSD<18%. The other is SPE method. LOQ is 0.05μg/L, the recoveries are between 78%-105%, RSD<15%. The direct sample method is a fast method and LOQ of the SPE method is lower.For aquatic products, such as fish, snail genus eriocheir, we develop two sample preparation methods. One is SPE method. LOQ is 0.5μg/kg, the recoveries are between 75%-106%, RSD<20%. The other is MSPD method. LOQ is 2.5μg/kg, the recoveries are between 73%-93%, RSD<16%. Similarly, there are two preparation methods for spires. One is SPE method. LOQ is 2μg/kg, the recoveries are between 72%-91%, RSD<18%. The other is MSPD method. LOQ is 10 pg/kg, the recoveries are between 78%-102%, RSD<13%. But the preparation method for spires is not same for aquatic products. The SPE method take time but the LOQ is lower. The MSPD HPLC-MSMS method can complete determination of seven MCs in an hour. The characteristics of speediness, high flux are not found in literature.