| Chemiluminescence (CL) was defined as electromagnetic radiation of the reactant, the production or the fluorescent substances as a result of a chemical reaction. The CL analytical method was established based on the moment radiation intensity or the gross radiation quantities.Chemiluminescence analysis has received much attention for the analysis of organic and inorganic species in a variety of fields for its high sensitivity, wide dynamic range and inexpensive instrumentation. Current research in CL analysis is focused on two general directions. One direction is to discover new CL reactions and broaden the analytical scope of CL analysis. The other direction is to combine the established CL systems with newly developing techniques, such as high performance liquid chromatograph and capillary electrophoresis, to increase the applied value of CL method. CL analysis has attracted much attention for its intrinsic advantages. CL analysis usually needs to employ CL reagent. The traditional CL reagent can be classified into two kinds: One kind of CL reagents is translated into the luminant in the reaction and the luminant could absorb the energy of the reaction to produce CL. The representative of this kind of CL reagents includes luminol, lucigenin and tris (2, 2'-bipyridyl) ruthenium (Ⅱ), etc. The other kind of CL reagents, such as peroxyoxalate can react with the oxidant to produce an intermediate. The intermediate formed a complex with a fluorescent molecule. When the complex decomposes, the fluorescent molecule is in its excited state. It comes back to the ground state accompanied with CL. In our recent investigations, it was found that fluorescent reagents may be considered to be another kind of CL reagents and could absorb the energy of the reaction to produce CL. Morin, a common fluorescent reagent, was widely applied in fluorometric analysis of some metal ions. However, there have been few papers with the intention of using morin as CL reagents in the CL analysis. Based on it, we explored the possibility of using morin as CL reagents in the CL analysis.This thesis consists of review and research section.In the first part, the CL was introduced briefly, the chemiluminescence system was summed up and the nature of morin and research applications was summed up, too.The research section consists of four parts.Part one, an investigation on chemiluminescence reaction of bromhexine hydrochloride in the cerium (Ⅳ) - morin system: we proposed a simple flow injection chemiluminescence (CL) method for the determination of bromhexine hydrochloride. This method was was based on the reaction of bromhexine hydrochloride with morin and cerium (Ⅳ) in acidic medium. The CL signal was proportional to the bromhexine hydrochloride concentration in the range 2.0×10-9 -2.0×10-7 g/mL. The detection limit was 9×10-10g/mL bromhexine hydrochloride and the relative standard deviation was 1.0 % (c=2.0×10-8 g/mL bromhexine hydrochloride, n = 11). The proposed method was successfully applied to the determination of bromhexine hydrochloride in the pharmaceutical preparations and human urine samples with satisfactory results. The possible mechanism of the CL reaction was proposed via the investigation of the CL kinetic characteristics, the CL spectrum and the fluorescence spectra of some related substances.Part two, an investigation on chemiluminescence reaction of mosapride citrate in the cerium (Ⅳ)- morin system: we proposed a simple flow injection chemiluminescence (CL) method for the determination of mosapride citrate. This method was was based on the reaction of mosapride citrate with morin and cerium (Ⅳ) in acidic medium. The CL signal was proportional to the concentration in the range 2.0×10-8 to 2.0×10-6g/mL. The detection limit was 9×10-9g/mL mosapride citrate and the relative standard deviation was 1.7 % (c=2.0×10-7g/mL mosapride citrate, n = 11). The proposed method was successfully applied to the determination of mosapride citrate in the pharmaceutical preparations and human plasma samples with satisfactory results. The possible mechanism of the CL reaction was proposed via the investigation of the CL kinetic characteristics, the CL spectrum and the fluorescence spectra of some related substances.Part three, an investigation on chemiluminescence reaction of phenolics in the KMnO4-morin system: CL signal was produced, when four phenolics, namely, phloroglucinol, 2,4-dichlorophenol, phenol and m-cresol reacted with morin and KMnO4. Based on these observations, a new flow injection CL method was developed for the determination of above-mentioned substance. The linear ranges of the method were 8.0×10-8-8.0×10-6g/mL phloroglucinol, 4.0×10-7-1.0×10-5g/mL 2, 4-dichlorophenol, 1.0×10-7-2.0×10-6g/mL phenol and 1.0×10-7-2.0×10-6g/mL m-cresol. The detection limits were 4×10-8g/mL phloroglucinol, 3×10-7g/mL 2, 4-dichlorophenol, 9×10-8g/mL phenol and 6×110-8g/mL m-cresol. The relative standard deviations (RSD) in 11 repeated measurements were 2.3 % (c=8.0×10-7g/mL phloroglucinol, n=11), 2.2 % (c=2.0×10-6g/mL 2,4 -dichlorophenol, n=11), 1.7% (c=6.0×10-7g/mL phenol, n= 11), 2.2 % (c=6.0×10-7g/mL m-cresol, n=11). The proposed method was successfully applied to the determination of phloroglucinol in wine and drinking water samples. The CL reaction mechanism was also discussed briefly.Part four, an investigation on chemiluminescence reaction of metal ions in the NBS-morin system: when Fe3+ and Ni2+ reacted with morin and NBS, CL signal was discovered. Based on this reaction, a new flow injection chemiluminescence system for the determination of Fe3+ and Ni2+ were proposed. The linear ranges of the method were 6.0×10-8-2.0×10-6g/mL Fe3+ and 5.0×10-8-1.0×109-6)g/mLNi2+. The detection limits were 3×10-8g/mL Fe3+ and 3×10-8g/mL Ni2+. The relative standard deviations (RSD) in 11 repeated measurements were 2.0 % (c=4.0×10-7g/mL Fe3+, n=11), 2.2%(c=2.0×10-7g/mL Ni2+, n=11). |
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