Juice Drinks Wastewater Anaerobic Fermentation Of Biological Hydrogen Production Technology Base Research

The biological technology of hydrogen production is an novel bio-energy production technology. Many organic waste was reduced while bio-energy was produced. The technology played a great role in energy crisis and environment deterioration. And it is beneficial to constant development and circular economy. It was preferential for its advantages, such as high efficiency, good stability and recycle capability.At present, the technology of bio-hydrogen production by anaerobic fermentation was only studied in laboratory. The matrixes were abundant, while, only single compounds and few multi-compound were adopted in present studies. In last years, soft beverage industry have made great progresses and its output reached 30.8696 million tons in 2005, in which juice was about 17.17%, equal to 5.30 million tons, furthermore, the increasing speed remained 20% or so. Soft beverage has become one of the most prosperous industry in food manufacture. In the paper, wastewater from juice manufacture was adopted and influential factors of bio-hydrogen production, such as sludge-pretreatment, main ecological factors and metal ion, were mainly studied.Sludge pretreatment was one of important factors in bio-hydrogen production by anaerobic fermentation. The effects of heat, acid and alkali were investigated respectively. It was proved heat was prior to the others, for it could killed methanogenic bacteria and promoted bio-hydrogen production.pH was also one of important factors in bio-hydrogen production by anaerobic fermentation. Both hydrogen output and concentration were the highest, when initial pH was 7.0.and it was showed pH was the perfect condition when it varied at the range of 4.5～5.5.Temperature played a great bio-hydrogen production by anaerobic fermentation too. Cumulative hydrogen was the most when temperature reached 32°C, and the rate of bio-hydrogen production was the highest when temperature was 37°C..Fe and Fe²⁺ was beneficial to the acceleration of bacterial metabolism and the promotion of activition of enzyme of bio-hydrogen production. while Fe³⁺ was of no effect. It was proved Fe²⁺ was perfect, whose promoting effects were relative to its concentration. Bio-hydrogen production was promoted, when [Fe²⁺] was 0～250mg/L ,while, it was restrained when [Fe²⁺] was higher than 250mg/L.when [Fe²⁺] was 50mg/L, corresponding cumulative hydrogen, 1936mL and concentration were gotten., which was improved 0.15 and 2.4% individually than the blank one.The same experimental conditions in different seasons were controlled, corresponding experimental results showed the natural temperature had an important influence on the activity of hydrogen-production of sludge. And higher temperature was favor for improving the activity of hydrogen-production.In the paper, the former experimental results were validated by series experiments. It was proved that the technology of bio-hydrogen production by anaerobic fermentation was applied in treatment of wastewater from juice manufacture was feasible. The last results, concentration of hydrogen 43.6% and maximal hydrogen 1960mL/L(which was equal to 2.45m³H₂/m³ waste water) were achieved and were higher 4.1% than the similar study reports at home and the average hydrogen-production reached 22.4 mmolH₂/g(COD).