Polysaccharides In The Pulp And Waste

The HSAL effluent of Yunnan industrial hemp stalk contains abundantly lignin and polysaccharides. The lignin research can be processed and analyzed by the traditional method;however, the polysaccharides research has not been reported and published currently, except the master graduation paper whose author is Xu Hengqi. This study developed the basis of Xu' s, and established both phenol-sulfuric acid method and Bradford method so as to determine polysaccharides quantity as well as protein quantity contained in HSAL' s effluent at each following step of this experiment: extraction, separation, and purification. Consequently, final polysaccharides components of BP, PAM, MPB and SP4 are obtained and, subsequently, are analyzed by IR and GC.This experimental separation procedure for polysaccharides from HSAL effluent is that the pulping effluent after filtration, centrifugation, and concentration, is purified by anhydrous alcohol to remove lignin, by Saveage method to separate protein, and by ether accompanying with acetone-washing, and then dialysis process to get rid of impurities. The optimal procedure conditions of ether extraction are: 1)The proportion of the effluent volume and ether volume is 1:1;2) Stirring time is 1 h;3) Maintenance time is 5 h.By applying GPC method for purification and classification of crude polysaccharides, this experiment gains four kinds of polysaccharides groups. They are BP, PAM, MPB and SP, respectivelt. And their molecular weights are following below: BP = 38753 Da;MPA = 37308 Da;MPB = 6790 Da;SP = 5715 Da. GPC s optimal operation parameters are following below: 1) solution concentration is 10mg/ml 2) solution volume/ gel bed volume is 2%;3) elution rate is 0. 25ml/min.Both IR and GC analysis of BP, PAM, MPB and SP polysaccharides show that BP contains xylan with rhamenosyl, arabinosyl, glucosyl, galactosyl residues (the mole ratio is that Xyl:Rha:Ara:Glu:Gal = 1.00:0.31:0.50:0.32:1.46);And MPA contains xylose, arabinose, glucose, galactose, fructose at least, (themole ratio is that Xyl:Rha:Ara:Glu:Gal:Fru = 1.00:0.14:0.22:0.20:0.90:0.12);And MPB contains xylose, rharaenose, arabinose, galactose, and fructose at least, (the mole ratio is that Xyl:Rha:Ara:Gal:Fru = 1.00:0.05:0.08:0.29:0.16);And SP contains xylose, rhamenose, arabinose, galactose and fructose at least, (the molecule ratio is that Xyl:Rha:Ara:Gal:Fru = 1.00:0.09:0.05:0.15:0.21. Additionally, IR shows that there is no mannose and glycuronic acid, and that probably there exists some lignin in MPA, and that perhaps, there are N-acetoamido glucosyl and N-acetoamido galacosyl groups in MPB. SP polysaccharides has three types of polysaccharide bond they are l-*6 (or nondexidized terminal) -. 1—4 (2) and l-*3, and the proportion is 0.25 : 0.20 : 0.55.