| High Performance Liquid Chromatography (HPLC) as an important modern measurement of separation now is one of high performance and fastly speed analysis and separation technology emerging in the 1970's. Due to its' convenience, high-speed, sensitivity and fairly accuracy, it has been widely used in the areas of production of medicine, foodstuff, environment etc.In this thesis, several reversed-phase HPLC methods of analyzing different organic compounds have been established, Including: (1) directly quantitative determination the impurities content of ionic liquids with reversed-phase HPLC. Proper analysis condition and standardized curves regression equation by external standard method was established. Meanswhile, linear range, and correlation coefficient(r), Relative standard deviation was determinated. (2) Ionic liquid was frist used as UV-proble in high performance liquid chromatography (HPLC) mobile phase in the quantitative detection of several alcohols. The influence of types and concentration of ionic liquids, detection wavelength, proportion of mobile phase, flow rate on the result of detection were discussed. We discovered that detection sensitivity can be increased tremendously through choosing different ionic liquids, because both positive and negative ions have great effected on sensitivity, so using ionic liquids as UV probes for indirect photometric chromatography is superior to the originalUV-probes. (3)Ionic liquids— tetrabutylammonium tosylate ([TBA][TOS]) is utilized as UV-probe in high performance liquid chromatography (HPLC) mobile phase for the detection of organic tin compounds. A rapid and convenient method of analysis of tributylorganotin compounds is established. (4) A resolution of Tamsulosin hydrochloride and intermediates and derivatives of mandelic acid by reversed-phase HPLC was set up by using reversed-phase on chiralcel OD-R, a polysaccharide-derived loading material—chiral stationary phase, adjustingmobile phase with different proportion of sodium perchlorate monohydrate solution and acetonitrile. The regularity of separation of enantiomers of mandelic acid derivatives by reversed-phase chiral HPLC is discussed. It was found that the mandelic acid esters linked up long carbon chains can be easier separated than that of short carbon chains. It was concluded that the chiral center of mandelic acid esters with long carbon chains has been inducted more intensively by the chiral center of stationary phase. |
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