Antibody Single Monolayers And Sensor Performance

In　this　paper,　a　method-immobilized　antibody　modified　with　self-assembled　monolayer　(SAM)　has　been　described　by　means　of　covalent　binding　with　1-ethyl-3-[3-(dimethylamino)propyl]　carbodiimide　hydrochloride　(EDC)　or　glutaraldehyde.　The　preparation　of　the　immunosensor　is　described　as　followed:　1.The　electrochemical　behavior　of　gold　planar　electrode　,silver　disk　electrode,　gold　disk　electrode　has　been　investigated.　They　were　used　to　fabricate　the　immunosensor,　respectively.　It　shows　that　the　immunosensor　using　gold　planar　is　more　sensitive　and　more　stable.　2.　The　electrochemical　behavior　of　three　different　SAM　[SH(CH2)10COOH　,EG6OCH2COOH　and　cysteine　]electrodes　has　been　investigated.It　shows　these　three　thiol　were　successfully　immobilized　on　the　gold　planar　because　the　potential　change　great.Different　thiol　were　used　to　fabricate　the　immunosensor,　It　shows　that　the　immunosensor　using　SH(CH₂)₁₀COOH　has　a　larger　detection　range　and　a　better　linear　relationship.　　3.　The　suitable　temperature　and　pH　to　prepare　of　the　immunosensor　recorded　in　literature　is　30℃　and　pH=5.5.　　4.The　response　of　the　immunosensor　to　mouse　IgG　at　different　temperature　and　pH　has　been　determined.　It　shows　the　suitable　temperature　and　pH　for　immunoreactions　is　30℃and　pH=5.5.　5.The　characteristics　of　the　immunosensor　using　SH(CH₂)₁₀COOH　have　been　investigated　in　detail.　It　can　detect　the　target　molecule　in　the　range　of　0.4-12ng/mL.　The　detection　limit　to　mIgG　is　0.4ng/mL,　the　correlation　coefficient　is　0.9912,the　response　time　is　12　min,　the　immunosensor　can　be　regenerated　using　0.04mol.L-1HCl　and　its　lifetime　is　16　days.　6.The　characteristics　of　the　immunosensor　using　cysteine　have　been　investigated　in　detail.　It　can　detect　the　target　molecule　in　the　range　of　0.05-5ng/mL,　The　detection　limit　to　mIgG　is　0.05ng/mL,　the　correlation　coefficient　is　0.9931,the　response　time　is　14　min,　the　immunosensor　can　be　regenerated　using　glycin-HCl　buffer　(pH2.3).