Ainsliaea fragrans Champ, is plant of Compositae. It has the effects of clearing heat and detoxification resolving dampness and arresting bleeding. The research of contemporary pharmacology shows that the coarse extraction of A. fragrans Champ , can inhibit of stphylococcusaureus and beta streptococcus. Purposes of this thesis are to research the active constituents of A. fragrans Champ, and establish the basis for exploring and utilizing it for the future. It may be summarized as follows:(1) Reviewing the study progress of the researches of Ainsliaea pharmacognosy and A. fragrans Champ, identification of the botanic origins chemical constituents pharmacological action and clinical treatment. And the paper has reviewed the recent application of high-speed countercurrent chromatography in the separation of natural products.(2) As for the chemical constituents of this plant, systemic studies lead to the isolation of three compounds from the extract, and two of them were identified. They are 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid. And they are isolated from Ainsliaea for the first time. By GC-MS and spectra searching database, twenty-four constituents of the volatile constituents from the extracted fraction were identified.(3) In this study , researching the technics of the reflux with alcohol water leaching and removing impurity by alcohol extraction by water and absorption by macroporous resin .Confirming the optimal extractive technics as extraction by water and absorption by macroporous resin, and the active components as total phenolic acids, that has the obviously function of antisepsis and anti-inflammation, combining the screening according to the pharmacological effects.(4) Determining the content of total phenolic acids in A. fragrans Champ, and the coarse extraction of A. fragrans Champ, over 50%,by UV spectrophotometer and using the 4, 5-dicaffeoylquinic acid as standard .Primarily analyzing the constituents of extraction by HPLC-MS.That can establish the basis for benefiting utilizing and developing A. fragrans Champ, and the coarse extraction of A. fragrans Champ. |