Complex Physical Field Mutagenic Effect Of Micro-organisms

Microbial breeding selection with physical mutagens was investigated in this paper. The strain WB-2 of high yield cellulase was the mutant of strain LBT-3.6 treated by UV and microwave, its filter paper activity was increased by 202.7%. The optimum parameters for fermentation consisted of 50g/L wheat bran, 0.4% (NH4)3PO4, 0.1% peptone, 0.4% NaCl, 0.2% Tween80, pH8.0. The maximum FPA of WB-2 reached 12.35U/mL after growth at 30癈 for 48h on a rotary flask shaker. Effect on growth of WB-2 was studied using five LEDs, which contained red, yellow, blue, green and purple.The strain LZ25-11 of high yield biosurfactant was obtained after UV treatment from L-21, its output of glycolipid was increased by 112.5%.