Liquid paraffin can be transformed to Long chain dicarboxylic acid, Biosurfactant and Single Cell Protein, which have high additional value. A strain of Candida was isolated from wastes silt of the petroleum by a series of steps including hydrocarbon enriching, TLC etc, which can produce dicarboxylic acid.It also can produce another matter while producing dicarboxylic acid, which optimized yield-1.430g/L is more than the yield of dicarboxylic acid. It can decreases water's surface tension from 72.3 to 32.5mN/m. Through a series of laboratory experiments it is shown that this matter can maintain highly surface active in wide pH value range(l-12), saturated NaCl salts and the temperature of 121℃.It is distinguinished to be lipopeptide biosuifactant by TLC IR. GC/MS. And its CMC value is 150mg/L.It's hoped that this biosurfactant be used in enhanced oil production and recovery. |