Extraction Of Fermentation And Separation Of Fulvic Acid (fa)

The technology of solid state fermentation and extraction of Fulvic acid with apple pomace has been studied. The optimized material ratio of apple pomace to bean cake to bran was 50:4:3 containing water up to 20% by the orthogonal experiments and two-step solid state fermentation was used for Fulvic acid production. First, Trichoderma viride and Trichoderma Koningii were inoculated respectively in the ratio of 2.5% in the meantime. The solid substrates were fermented in pH=6.2 at 28 ℃ for 12d and then exterminated. Secondly, Candida spp, Saccharo spp, Bacillus subtilis and Corynebacterium spp were inoculated respectively in the ratio of 2.5% at the same time. The solid substrates were incubated at 32℃ for 12d. The yield of fulvic acid reached to 41.2% under the optimized conditions.The orthogonal experiment showed that the optimum conditions of extraction from fermenting products were that density of sodium hydroxide concentration is 3.0%, extracted at 60℃ for 1.5h, then adjusted acidity with hydrochloric acid to pH=2.0, the purity of Fulvic acid was 45.8%. Fulvic acid was further extracted from alkaline extract with potassium permanganate solution in order to oxidate degradation under optimized conditions of the ratio of 0.25mol/l potassium permanganate solution to sodium humate solution by 1:6.25 (voluminal ratio), pH=12, at 85℃,oxidative degradation for 0.5h, the yield of Fulvie acid was 1.12g/l.Solid state fermentation of Fulvic acid with apple pomace as the main raw material was fast, doable and economical method.