The Electrochemical Behavior Of The Effective Ingredients Of Chinese Herbal Analgesic Wild Papaya Glycoside

Chinese traditional and herbal drug ( CTHD) is the civilizing treasure of our countrv. Now. there are about several thousands kinds of CTHD that have been found from plants, animals and minerals. However, Because of the complexity of CTHD, There is very difficult in the investigation of physcilogicial function , curative effect and basic study. Of course ,It must baffle the spread of its application. In this paper, we try to use electrochemistry technique to study the electrochemical behavior of CTHD and summarize the instructive experience in using electrochemical technique to investigate the medicinal mechanism of CTHD.In this thesis, we try our best to apply cyclic voltammogram (CV) and electrochemical impedance spectroscope (EIS) to disclose Phenazine methosulfate (PMS) modified gold elctrode and its electrocatalytic activity for oxidation of NADH. In this basis , We study the electrochemical behaviors of Yemuside (YMS) that is purified from Stauntonia chinensis(DC) at bare gold electrode and the lipid bilayer-electrode incorporated with YMS; and talk over respectively the electron transfer of YMS in different environment.The experimental results show that the reversibility of PMS incorporated in BLM/Au electrode is better than at the bare electrode surface .The oxidational current of NADH increases largely at the lipid bilayer-electrode incorporated PMS , at the same time , the oxiditional potential of NADH obviously shifts to negative orientation. It is possible that the bilayer lipid membrane is close to the true biologic membrane, It provides a micro-environment which is very approachable biologic system , which is favorable for PMS to catalyze the oxidation of NADH. We study the electrocatalytic oxidation of NADH .The electrochemical behavior of YMS on the bare gold electrode and Au/BLM were investigated by CV and EIS. The results showed that the electron-transfer of YMS was an irreversible process and mainly controlled by diffusion on bare electrode . However, the electron-transfer of YMS is a quasi-reversible process at BLM/Au electrode. That is, the electron transfer on YMS-BLM/Au electrode waspromoted. From EIS, we simulated their corresponding equivalent circuits and analysed . The electron-transfer apparent resistance of YMS bare electrode and YMS modified in BLM/Au electrode were respectivelyl48.67±7.56 Kohm and 25.62±4.69 Kohmi the latter decreases ; while the electron-transfer rate : the former is (3.59 ± 0.52) ×10-6 cm s-1, the latter is only (2.08 ± 0.45)×10-4 cm s-1. It proves that the electron-transfer rate of YMS incorporated in BLM is obviously quickened . The possible explanations : first, the amphiphilic structure of BLM can act as a "filter", which can inhibit adsorption of macromolecules on the surface of electrode. Second, YMS incorporated in BLM is affected by the amphiphilic structure of BLM and is rearrayed in structure. This is benefit for the electroactive center of YMS is very close to electrode. Last, BLM also provides the micro-environment for YMS.From the above experimental results, electrochemical technique can provide the available information in studying Chinese traditional and herbal drug.