| A water soluble crude polysaccharide has been isolated from the Hericium caput-medusae . HP and HP were obtained by sepharose-CL 6B column chromatography gradation and examined homogenous by sephadex G-100 column chromatography and cellulose acetate film electrophoresis .P.C. and G.C. analysis indicated that HP I was composed of Fuc, Glc, and Gal in the molar ratio of 0.423: 1: 2.110, and HP II was composed of Glc and Gal in the molar ratio of 11.529: 1.In the structure analysis of HP and HP , partial hydrolysis with acid, periodate oxidation, Smith degradation, I.R., G.C., methylation and GC-MS analysis were the principal methods used.The results revealed HP I was a kind of highly branched structure. The main chain is made up of (1-6)-linked-Gal and is substituted at 0-2. On average, there is 1 branch among 3 main chain residues. The side chain is composed of-1Fuc4-, - (-1Glc3-) 2-and - (-1Glc9-) 3-; The nonreduced end is composed of Glc and Gal.The results revealed HP was a kind of highly branched structure. The main chain is made up of (1--6)-linked-Glc and is substituted at 0-3. On average, there are 3 branches among 7 main chain residues. The side chain is composed of - (-1Glc3-)2- and -1Gal6-; The nonreduced end is composed of Glc.n1-17 n2=14... |
