| As a refined plant oil, domestic and abroad scientists found that Zanthoxylum oil which extracted from zanthoxylum bungednum, belong to zanthoxylum of retraced, had quite powerful and different biological active to grain pests (Such as bungeanum sitotroga cerealella, sitophilus zeamains,, Tribolium confusum, Tribolium castaneum and Callosobruchus maculatus ) and many mildew (such as Aspergillus sp. andpenicillium sp.). By studying on its extract, biological activity and some physiological reaction on certain farmland pests, forest pests, plant pathogenic and healthy bacteria's, it showed that:1 immature fruit had higher content of zanthoxylum oil than others, with the growing of fruit, the centent of zanthoxylum oil began to decrease; Fresh fruit had higher collect efficiency than dry fruit; By using the method of water vapor cycle distillation, the abstract efficiency of refined oil can be improver extremely.2 Zanthoxylum oil had poorer bacteriostatic action effect on fungus than germs. Experiment showed that mildews was subjected to be more controlled by zanthoxylum oil than yeast. G germs was subjected to be more controlled by zanthoxylum oil than G+ germs; Above the effective concentration, the control effect by zanthoxylum oil on bacterium and fungus was increased with the improvement of the oil concentration; Below the effective concentration the controller effect was constant with the change of the oil concentration. Concentration variation of zanthoxylum oil had the most effective on fungus. However, both bacterium and fungus had been powerful restrained at 49h.3 Biological active experiment of zanthoxylum oil on pests showed that:(1) Zanthoxylum oil and quite powerful fumigation action effect on Plutella maculi pennis, Mythimna separata and Leucoma Candida, the LDso of the three kinds pest were 6.7491 u 1/L, 8.3568 u 1/L and 6.4508 u 1/L respectively. Suffocating active and time had a hotable direct relationship;(2) Zanthoxylum oil had fairly contact effect on Mythimna Separata and Leucoma Candidq, the LD50 of the two kinds pest were 5.2555 y 1/L and 1.1692 u 1/L; (3)Zanthoxylum oil had exceeding powerful resist food effect on Leucoma Candida,howere, with the extend of time, the seduce food effect began to appear for Mythimna Separata; When the concentration of zanthoxylum oil below 0.05ml/ml, both Leucoma Candida and Mythimna Separata showed a fluctuate resist food effect from.(4) Zanthoxylum oil had quite strong abstain from Mythimna Spearata and Leucoma Candida,\viih the extend of time, the avoid rate were began to decrease.4 The poisoning symptoms of experimental pests, which had been dialed with zanthoxylum oil (19.35 u 1/L and 15.48 u 1/L), were slow in action, spit out water, lose water on body surface, without excitement and convulsion, of which belong to the respiredpoisoning symptoms. However, the poisoning symptoms of Leucoma Candida, which had been dealed with zanthoxylum oil (19.35 p 1/L) were fast in action and run around, after a short while, the experimental pests were weaken inaction, totally paralyzed and twitched, at last, the pest's body was wrinkled and died.5 Effect of zanthoxylum oil on experimental pest's biology and physiology showed that:(1) Zanthoxylum oil had striking influence on the respiratory system of Mythimna Separata laboratory test showed that zanthoxylum oil can lead to the increase of oxygen consumption of experimental pests, which restrain their oxidants capable.(2) The protein content of hemolymph lymph in experimental pests, which had been dialed with zanthoxylum oil, was decreased obviously comparing to common pests but the protein content of organize liquid was higher than common pests.(3)The effects on expression of estterase isoenzyme which were in hemolymph and tissue fluid of Leucoma Candida which were four ages by contact application.whole studying showed that zanthoxylum oil had many effect ways on pests, which effected the pest's harrows and respiratory systems, and also have some potential effect patterns on... |
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