Phosphate Type Surfactant Synthesis, Characterization, And Reverse Micelle System Lipase-catalyzed Hydrolysis Reaction

With the development of biotechnology and material science, reverse micelles have been focused on internationally. It has become emergent to exploit new surfactants, which suit for constituting reverse micelles applied to enzymatic catalysis.For this purpose, we synthesized a new anionic surfactant, Sodium di-[(2-ethylhexyloxy) ethyl] phosphate ( DEEPA for short). Its structure has been identified by !H-NMR as well as IR spectrum. Its physico-chemical properties, such as cmc ( critical micelle concentration), HLB ( hydrophile-lipophile balance) value, and its ability of solublizing water were also measured. Results showed that DEEPA has a comparatively high surfactant activity.Camlicla Lipolytica Lipase was entrapped into reverse micelles composed of various surfactants, and activities of each system in hydrolyzing olive oil were examined. Contrasted with the activity of the same reaction in oil-water double phased system, the hydrolyzing behavior of Camlicla Lipolytica Lipase in reversed micellar systems is much like that in oil-water double phased system. The stability and catalyzing activity are both enhanced and give rise to 'superactivity'. except in reverse micelles composed of cationic surfactant.Finally, we discussed the effect of surfactant on hydrolysis catalyzed by reversed micellar lipase. For the specific reaction of Camlicla Lipolytica Lipase, anionic surfactants are superior to cationic surfactants and non-ionic surfactants. By discussing the relationship of similarly structured anionic surfactants and the activities of lipase entrapped into their reversed micellar systems, we get the point that both kinds, that aresulfuric and phosphoric surfactants, that their own advantages for lipase-catalyzed reactions in reverse micelles. The cause of this is also interpreted briefly.