Phosphate Sensors And Sensitive Reagents

The negative effects caused by seawater-supernutrition were surious. To monitor the accumulation and transfer of phosphate real-timely, sensitive apparatus was the key. During common methods, the process of beforehand disposition was overloaded with details, and said nothing of real time. Artd eletrochemical sensor biochemical sensors were no better, whose stability was so weak that it was unable to be used in the automatic detection. On the other hand, fluorescent optic sensors were of perfect sensitivity and selection, and the derivation of acyl-amine sensitive to phosphate was a ideal reagent that was easy to synthesis and sensitive to trace phosphate in visible region from 400nm~550nm( A.) and had an ability to remain optic sensor reversible.Therefore, fluorescent sensitive membrances were studied to solve the problems, and new sensitive reagents were made to prepare some stabile sensitive optic sensor to monitor the water quality real-timely.In this paper, two kinds of Al-Morin sensitive membranes had been developed. With a technology to form a sandwich configuration of the poly (vinyl alcohol) PVA and poly (vinyl chlorine) PVC composite membrane, and the mathematic analysis model Io/I=(K.w Ke)/ Kf [M], a stable fluorescent sensor based on Al-Morin for the measurement of phosphate was developed. Stored in a dry condition, sensitive membranes were stable for 90-120 days, and in aqueous solutions with a pH value of 4.0 and a salinity ranging from 20.0 to 30.0, PVA/PVC membranes were transparent and pliable, a calibration graph was obtained in the range of tPCV from 1.50 to 7.00 ug/ml with the limit of determination was 0.02 ug/ml, the response time of the equilibrium for trace phosphate was less than 5 min. Marinated in 1.0 X 10-3 mol/L Al-Morin solution for 3-5min to supply fluorescent indicator, and then washed with redistilled water, the composite membrane remained a constant concentration of Al-Morin indicator, and could be used for 50-80 degrees reversibly. In addition, the relative error of recoveries of HiPCV ranged from-3.3 to +2.1%, and some coexisted anions had little interference relatively.Besides, by abstracting Al-Morin from acid solutions into organism with Pentyl alcohol to form a fluorescent PVC Sensing membrane, a new study has been developed to measure phosphate in seawater. It is found that in the buffer solution ( pH=5.0), calibrationgraph of FPCV was obtained in the concentration that ranged 6.00-15.00 U g/mL and the detective limit was 0.02 n g/mL. Response time was less than 450s, and the recoveries range from 96.60% to 103.81%. With several supplements, the PVC sensing membrane can be applied for 60 degrees.In this paper, the preparation of a new sample amide functionalized ferrocene derivative was prepared, and its anion recognition properties were investigated, in which a p-Nitroaniline group was linked to a H-bonding amide (CO-NH) to make a new study of anion sensor. In this type of donor-acceptor, a charge transfer mostly accompanies the electronic excitation from donor nitrogen to acceptor substituent (-NC^). Thus, the excited state would be more strongly stabilized, resulting in a color enhancement. It could act as both hydrogen-bond donors and acceptors, tuning the recognition process and leading to novel phosphate selectivity. By the reaction of p-Nitroaniline with l,l'-Ferrocenedicarbony chloride, a new neutral Ferrocenoyl receptor l,r-Bis[((p-nitrenilinyl)- amino) carbonyl] -ferrocence) has been developed and characterized by IR, UV visible spectrum, DTA and Element Analysis to make systems analysis for studying the selective sensing of phosphate. The result shows that at 420nm, the new neutral Ferrocenoyl receptor were stable and sensitive to phosphate dissolved in water, and its calibration graph of PO43"is obtained in the concentration that ranges form 6.60 to 54.50 u g/mL and 1.00 to 10.00 u g/mL , the detective limit was 0.003 u g/mL and O.Olug/mL respetively.