Thirty-seven Isolation, Purification, And Immune Activity

The polysaccharides PNPSlIa and lib were isolated and purified from Panax notogisen Burk F.H.Chen, a Chinese traditional medical plant. The polysaccharides were extracted with hot water and precipitated by alcohol. Protein was removed by Sevag method combined with neutral enzyme. Then the products were further purified with column chromatography on DEAE-Sepharose Fast Flow and CM-Sepharose Fast Flow and Sephadex G-200 orderly. The PNPSII b was identified as homogeneous by Sephadex G-200 and determination of specific rotatory power. Infrared ray spectrum indicates there are typical characteristic absorption peaks of polysaccharides. The sugar components of PNPS II b were Ala, GIu and Gal. Its molecular weight estimated by GPC was 20,700.The effects of PNPS II a and II b on the function of mice lymphocyte were studied in vitro and in vivo. PNPS II a and II b had significant enhancement of spleen proliferation alone at a series of low concentrations. But at high concentrations, the inhibitory effects were shown. PNPS II a and II b also had enhancing effect on lymphocyte proliferation of spleen with PHA. Analysis of the cell cycle proved the regulatory function of PNPS lib. PNPS lib can promoted the interleukin 2 (IL-2) secretion of spleen.The results showed that PNPS II considerably recovered lymphocyte function in the immunosupressing mice induced by cyclophosphamide. The inhibition function of ISO and DEX on lymphocyte proliferation was decreased with PNPS II b at specific concentration. It was concluded that PNPS II b was a kind of polysaccharide which had the immunoregulating function.