Gold Nanoparticles Modified Electrode In The Bio-electrochemical Applications

It is one of the trends of chemistry to use electrochemical methods in studying biological phenomena. Protein in organism is the macromolecular containing several ligands, which has important biological function. Application of nanometer materials has made great progress in modified electrodes. Nanometer gold(NG) particles exhibit noteworthy properties, such as the surface charge, biocompatiblility and large surface, etc. It抯 the focus of this thesis to study the characteristic of nano-gold modified electrode on the field of bioelectrochemistry. First, we focused our attention on the interaction of tris( 1,1 O-phenanthroline) Cobalt(IJJ) with BSA. Bovine Serum Albumin(BSA) were electrochemically immobilized on the surface of nano-gold modified glassy carbon electrode. The interaction of BSA with Tris(l,1O? phenanthroline) Cobalt(III) was investigated by the cyclic voltammetric behavior of Co(phen)33~ absorbed on the immobilezed BSA. The result suggests that the interaction is strong . A series of important data were obtained, such as binding ratio and interaction model and so on by cyclic voltammetry(CV), electrochemical quartz crystal microbalance(EQCM) and spectrochemical methods. To obtain information about the nano-gold electrodeposited on the glassy carbon electrode, XPS spectra were measured. Cyclic voltammetry(CV), different pulse voltammetry(DPV), chronocoulometry were used to study direct electrochemistry of cytochrome c. The results of CV and DPV showed that quasi-reversible electron transfer process occurred at the NG modified electrode and DNA modified electrode. Whereas chronocoulometry indicated cytochrome c was not absorbed on electrode surface. At last, DNA modified nanogold glassy carbon electrode (DNA/NGIGCE) was prepared by electrodeposition. The interaction of DNA with dopamine(DA),epinephrine(EP) and nor? epinephrine(NEP) was investigated by the cyclic voltammetric behavior of DA~. EP and NEP absorbed on the immobilezed DNA. Electrochemical quartz crystal microbalance(EQCM) was used to obtain information about the DNA electrodeposited on the nano-gold modified glassy carbon electrode . The result suggests that interactions are strong.