Drug Flow Injection Chemiluminescence Analysis And Its Application

Chemiluminescence analysis, based on the emission during some chemical reactions, is a technique for the determination of trace-amount species. Due to its high sensitivity, wide linear range, rapidity, simplicity and relatively low cost, chemiluminescence analysis has found application in assay of trace amounts of metal ions, inorganic anions, biological molecules and cancer-causing species, and in pharmaceutical chemistry, environmental science as well as clinical medicine. The thesis consists of two parts. The first one is a review dealing with the development of the application of chemiluminescence in pharmaceutical analysis. Emphasis is placed on the development in the recent five years. The other part is research reports. The kinetic properties of two chemiluminescence systems, K3Fe(CN)6uminol system and K3Fe(CN)6uminol4Fe(CN)6 system, were studied in detail. Various factors were also studied that have effect on the chemiluminescence intensity and/or on the ratio of signal to noise of the two systems. It was found that the aqueous solution of Vitamin K3 has depressing effect on the chemiluminescence intensity of the K3Fe(CN)6uminol system, while isoniazid has enhancing effect on it, and uric acid has enhancing effect on the CL intensity of the other system. Based on these discoveries, a series of experiments were conducted to optimize the conditions in point and to make clear the mechanisms of the three reactions. Sensitive, simple and fast methods were proposed for the determination of VK3, isoniazid and uric acid. A microdialysis samplinglow inj ectionhemiluminescence detection system was set up and has been successfully applied to the study of interaction between tetracycline hydrogen chloride and bovine serum album(BSA). It comprised several steps. Firstly, various factors of the K3Fe(CN)6uminol4Fe(CN)6 chemiluminescence system, such as oxidant concentration, flow rate and repressor concentration were studied in order to obtain optimum experiment conditions. As a result, a flow-injection chemiluminescence analysis method for the determination of tetracycline hydrogen chloride was developed. Secondly, the dialysis probe was calibrated and its recoveries at different perfusion rates were measured. Finally, the interaction between tetracycline hydrogen chloride and BSA was studied in vitro under the optimized conditions. The result compared well with that in the literature, indicating that the proposed system is suitable for the study of drugrotein interaction. A chemiluminescence (CL) flow system is described for the determination of menadione sodium bisulfite based on its repression on the chemiluminescence emission produced upon mixing a hexacyanoferrate(III) solution with an alkaline luminol solution in the absence of co-oxidizer. The system responds linearly to menadione sodium bisulfite concentration in the range 0 ?1 pg/mL with a detection limit (3cr) of 0.01 pg/mL. Relative standard deviation (RSD) of 0.16% for 0.4 pg/mL menadione sodium bisulfite (n1 1). The system has been successfully applied to the determination of menadione sodium bisulfite in tablets and injections. A chemiluminescence(CL) flow system is described for the determination of isoniazid based on its enhancement on the chemiluminescence emission produced upon mixing a hexacyanoferrate(III) solution with an alkaline luminol solution. The system responds linearly to isoniazid concentration in the range 0?1 pg/mL...