| Hydrating, antioxidant and sunscreen almost are the three topics in the field of skin care, because skin dehydration and ultraviolet radiation can cause great damage to the skin, and which are major causes of skin aging. Vitamin E has a strong antioxidant effect, at the same time, also has the function of the moist skin, however, it have been limited by its some characteristics, such as hydrophobic and frangibility to oxidation; As we all know, jojoba oil has highly moisturizing function, but the application have been limited by its some characteristics, for example coagulate easily under low temperature. At the same time, no matter in the field of skin care or drug delivery, there is a important problem need to be solved, which is drug or nutrients trans-dermal delivery. In this thesis, aiming at these shortcomings and problem, the micro-emulsion and micro-emulsion gel are studied. Jojoba oil is used as oil phase of micro-emulsion and micro-emulsion gel, and loaded vitamin E, which can protect and expanse the capacity of vitamin E, and also protect jojoba oil, at the same time, it can greatly improves the trans-dermal effect of vitamin E.The micro-emulsion formula screening, micro-emulsion and micro-emulsion gel preparation and its physical and chemical properties, stability, and in vitro trans-dermal release behavior are analyzed in this thesis. The main contents are as follows:(1)The optimum formula of blank micro-emulsion is screened preliminary by the pseudo-ternary phase diagram and stability property. According to the pseudo-ternary phase diagram of optimum formula, we take six points with different water content(10% to 60%) and prepare micro-emulsion respectively, and the physical and chemical properties are studied by variety of testing methods, the thermodynamics also is studied. The results are: all the micro-emulsions acing weak acid, its particle size are between 20 to 35 nm with uniform distribution, the surface tension are low, the refractive index also accords with the condition; The results of conductivity test show that the structure of micro-emulsion are gradually change with the increase of water content; The stability of micro-emulsion are good and the formation process was spontaneous.(2) The final optimum formula of vitamin E carrying micro-emulsion is optimized through central composite design method, according to the final formula, prepare micro-emulsion and micro-emulsion gel, and its physical and chemical properties, stability are studied and the water state in micro-emulsion are also studied. The results are: the ratio of oil and the mixture of emulsifier is 0.1, the water content of micro-emulsion is 44.439%, the vitamin E content is 5.856%, the retention amount of vitamin E in the skin is 683.331 micrograms; The micro-emulsion and micro-emulsion gel are both acting weak acid, particle size are within 10 to 20 nm with uniform distribution, surface tension and refractive index are accords with the blank micro-emulsion, the stability are both good. The oil-in-water structure micro-emulsion contains four different water states.(3) In vitro trans-dermal release experiments are did in Franz diffusion cell used the final micro-emulsion and micro-emulsion gel, the results show it can be concluded that both micro-emulsion and micro-emulsion gel were significantly can enhance vitamin E permeation through the skin. At the same time we prove the release experiments results through infrared spectrum, scanning electron microscope and CLSM. Combined with CLSM, scanning electron microscope and infrared spectrum study, all these studies results confirmed that permeation enhancement effect of micro-emulsion and micro-emulsion gel. |
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