Stydy On Preparation Technology Optimization Of Lentinus Edodes Peptide And Its Antioxidant Activity

Lentinula edodes is the second edible bacterium in the field of the world, is known as the “Shanzhen”. Lentinula edodes is a kind of high-protein low-fat and amino acid content more rich in edible bacterium. Because of high nutritional value and it has been welcomed. Many experts and scholars have pay attention to Lentinula edodes research, and less research on other ingredients, such as Lentinula edodes are lost as waste and resources underutilized, resulting in loss of a large number of high-quality protein, the use of extremely low, deep processing is not enough, the lack of high-tech and market potential of new products. Therefore, to improve the comprehensive utilization rate of mushrooms to achieve not only economic benefits, but also achieve high value. This experiment mainly of Lentinula edodes protein separation, Lentinula edodes peptide preparation, antioxidant activity on research and analysis.1. By alkali extraction and acid precipitation process for Lentinula edodes protein separation, with Lentinula edodes protein extraction rate as evaluation index, analyze the effect of different separation conditions on the extraction rate. The results showed that Lentinula edodes protein extraction rate of various factors from strong to weak sequential extraction time>pH>solid-liquid ratio>extraction temperature, alkaline extraction and acid precipitation parameters are: water ratio 40mL/g, temperature 50℃, 120 mins, pH 9.0. Separation of Lentinula edodes protein for subsequent digestion process.2. The Lentinula edodes peptide DPPH radical scavenging activity as the assessment object, and studies of enzyme solution temperature,time, pH and amount of enzyme scavenging ability of DPPH radical. Single factof tests and orthogonal test obtained for enzymolysis temperature of 50℃, 150 mins, pH 9.0, enzyme dosage 9000U/mL, scavenging ability of DPPH free radical was 61.7%. Lentinula edodes peptide is the highest activity under the conditions, the strongest antioxidant capacity.3. To obtain a strong antioxidant capacity Lentinula edodes peptides by ultrafiltration, dextran gel chromatography to separate and purify the peptides hydrolyzate research. The results showed that interception of 5KDa ultrafiltration continued separation, the molecular weight peptides solution was isolated by Sephadex gel chromatography, eluted the three peaks. After antioxidant activity assay, the peak Ⅲ eluent for the target component.4. Analyze the nature of Lentenula edodes peptide, amino acids can be measured by automatic acid analyzer variety, essential amino acids accounted for 41.5% of all the amion acid, it can be applied to the food nutrition fortifler. Application of matrix assisted laser desorption ionization time of flight mass spectrometry, the determination of the enzyme solution with Lentenula edodes peptide molecular weight under 3500 Da, the antioxidant activity is better.