Occurrence And Thermal Desorption Treatment Of Fluoroquinolone Antibiotics In Chicken Solid Wastes

As veterinary antibiotics, Fluoroquinolones had been extensively used in livestock farming industry for livestock disease control as well as feed additive. Most of the veterinary antibiotics ingested by livestock were not absorbed, but excreted with feces of livestock. Large amount of antibiotics entering the environment may lead to environmental risks including biological toxicity and pathogens resistance genes, and endanger environment and human health safety.In this research, six kinds of fluoroquinolones (FQs) antibiotics (Norfloxacin (NOR), Ciprofloxacin (CIP), Lomefloxacin (LOM), Danofloxacin (DAN), Enrofloxacin (ENR) and Sarafloxacin (SAR)) were studied, and a simultaneous detection analysis of multiple fluoroquinolone antibiotics in chicken feces based on the a solid phase extraction method, HPLC and fluorescence detection (SPE-LC-FLD) was established. The method was applied to investigate the pollution of FQs in livestock feces from five farms, removal efficiency and influencing factors of thermal desorption treatment technology to removal of FQs in livestock feces. The details are as follows:1. A simultaneous analysis method of six kinds of FQs in chicken feces based on the a solid phase extraction method, HPLC and fluorescence detection (SPE-LC-FLD) was established through processes of optimization of pre-treatment, extracting agent selection, extraction agent pH, which was fast, steady and had a high recovery rate and low limit of quantification. FQs showed a good linear relationship within concentration range of0.01～1.0mg/kg, and R2was0.9969to0.9999. Spiked recovery rate (concentration levels was0.01,0.5and1.0mg/kg dry weight) was76.7%～106.7%, and the relative standard deviation was0.7%～14.4%(n=4). The detection limit was0.002～0.022mg/kg, the limit of quantification method was0.0068～0.074mg/kg.2.The method was applied in the analysis of six FQs in chicken feces samples from4large-scale chicken farms and a natural stocking farm. Targets were detected at different levels in samples from4large-scale chicken farms, whereas no targets were detected in samples from a natural stocking farm. FQs concentrations were rangerd from ND to1.13mg/kg.3. In order to remove FQs from chicken feces. removal effect of thermal treatment technology to four FQs (Norfloxacin (NOR), Ciprofloxacin (CIP), Lomefloxacin (LOM) and Enrofloxacin (ENR)) in chicken feces was explored. Influence of different temperatures, different residence times and different moisture content to removal effect of FQs were studied. Results showed that removal rate of the four FQs increased with increase of treatment temperature and time. Removal rate of FQs in chicken feces reached55.7%～76.5%at190℃, at time of40min. Starting from190℃, chicken feces began to darken carbonation and FQs removal rate increased significantly. Within a certain range of moisture content (5%-25%) in chicken feces, the removal rate of FQs by process of the thermal treatment was not significant.