| In order to investigate the application capabilities of the thermophilic bacteria Geobacillus sp. ZY-10in microbial enhanced oil recovery and oil pollution bioremediation, uptake mode of the long-chain aliphatic alkanes and the biodegradation capabilities of the long-chain alkanes by strain ZY-10were studied.With xylose as carbon source, shake cultivation or static cultivation with CaCl2could improve cell growth. Incubation conditions could affect the cell morphology of strain ZY-10, the cell morphologys were observed under light microscope during6days’incubation. Most cells showed a straight rod in the early period of cultivation, with incubation extended the majority of cells showed a shorter rod under the condition of shake cultivation or static cultivation with CaCl2, under the condition of static cultivation or static cultivation with FeSO4most cells showed a longer rod, longer linear cells were observed in the culture after14days’incubation.With tridecane or hexatriacontane as carbon source, CaCl2could promote the strain to utilize tridecane and shake cultivation was not benefit for the strain to utilize tridecane. Strain utilized hexatriacontane difficultly. Under the incubation on tridecane as carbon source, the emulsification activity of the fermentation supernatant of strain was enhanced with incubation time extended, the emulsification activity reached the highest after12days’ incubation, the higher emulsification activity was observed with tridecane, lipuid paraffin, sunflower oil, gasoline, diesel fuel and motor oil, while the lower emulsification activity was observed with peanut oil, corn oil and soybean oil.Incubated on tridecane or eicosane as carbon source, the results showed that strain could rapidly utilize tridecane, residual rate of tridecane and eicosane were35.9%and59.9%after30days’incubation, respectively. Strain ZY-10might uptake tridecane by the mode of direct contact and biosurfactant-medium, which transported tridecane into the cell for metabolism. |
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