Effects Of La(â…¢) And Acid Rain On H~+-ATPase Activity In Leaves Of Rice Seedlings

Acid rain(AR) pollution has become a serious environmental pollution in the world, which severly affected agricultural and forest production. REEs has the biological effects "Low promote high suppression", it had been studied extensively in response plant resistance. In this paper, the plasma membrane H+-ATPase as the starting point for studying the relationship between the plasma membrane H+-ATPase activity and the resistance of REEs. In this paper, the effects of lanthanum and Acid Rain on membrane H+-ATPase activity in rice leaves were studied and it can provide basic data for the clear relationship between lanthanum and plant internal mechanism to acid resistance. The major results are shown as follows:In the stressed period, compared with the controls, the membrane permeability, content of intracellular H+, intracellular Ca2+, as well as H+-ATPase activities in rice leaves treated with p H 3.5 increased, the relative growth rate and ATP content are decreased. The membrane permeability and the content of intracellular H+ are increaded in group p H 2.5. Compared with the controls, 15 mg·L-1 La(La1) incresaed the activities of H+-ATPase, whereas, the opposite effects was observed in leaves treated with 30 mg·L-1 La(La2). Compare with siglnal rian, the group treatment with La1+ p H 3.5 the relative growth rate and ATP content are decreased in leaves. All index had no obvious change when treated with La1+ p H 2.5. All indexs were decread treated with La1+ AR except the membrane permeability. All indices were infeior to CK in p H 2.5 treatment, La1+ p H 2.5 treatment and La2+ AR treatments, other treatments were recovery.Compared with the controls, the set of genes OSA1, OSA2, OSA4, OSA7, OSA8, OSA10 increased expression of plasma membrane H+-ATPase activity increased in p H 3.5 treatment. The expression of each gene of group with p H 2.5 reduced. Gene OSA1, OSA2, OSA4, OSA7, OSA8, OSA10 expression of plasma membrane H+-ATPase activity of group with La1 increased. Only gene OSA4 expression group with La2 increased. Compared with p H 3.5 treatment, the set of genes OSA3, OSA9 expression levels increased in La1+ p H 3.5 treatment. Compared with Group with p H 2.5, the expression of genes OSA7 increased in group of La1+ p H 2.5. The set of La2+ p H 3.5 genes OSA2 increased, La2+ p H 2.5 group gene expression levels decreased. After restoring 5 d, compared with the stress phase, OSA1 and OSA2 expression decreased in p H 3.5 treatment. Plasma membrane H+-ATPase gene expression level with p H 2.5 treatment was still lower than CK, plasma membrane H+-ATPase activity was not restored. OSA1 and OSA7 expression levels in La1 treatment increased. Only OSA4 expression of the group with La2 of increased. Compared with the stress period, OSA2, OSA4 and OSA10 expression was reduced. La1+ p H 3.5 group with La1+ p H 2.5, only the expression of the gene OSA7 increased. The gene of expression plasma membrane H+-ATPase almost all dreased in La2+ AR treatment, leading to plasma membrane H+-ATPase activity decreaded.The plasma membrane IUFA decreased, MDA and H2O2 content increased stressed by acid rain compared with CK. The CAT activity of group with p H 3.5 increased, while decreased in group with p H 2.5. The membrane IUFA and CAT activity increased, MDA and H2O2 content of group with La1 decreased. Changes between La2 group and La1 group are opposite. Compared with signal acid rain treatment, the plasma membrane IUFA and the CAT activity of group with La1+ p H 3.5 were increased. Compared with p H 2.5, the indexes of group with La1+ p H 2.5 remained unchanged. The CAT activity and membrane IUFA of group with La2+ AR decreased, resulting in the plasma membrane H+-ATPase activity decreaded. Restore 5 days, the CAT activity of groups with p H 2.5,La1+ p H 2.5, La2+ AR was inhibited, plasma membrane IUFA reduced plasma membrane H+-ATPase activity was inhibited.