The Preparation Of Three Liposomes And Theirs Application In Cosmetics

The study of this dissertation is mainly about preparating conditions of liposome, obtaining great encapsulation and improving the content of active substances in lipsome formula, and applying to cosmetic. Because of the liposome can improve the disadvantages of some active substances, and itself has many excellent characters, so they are widely used in several of industries.The chloroform was used as solvent, preparation of liposome for encapsulating vitamin E. Extent of encapsulation was taken as evaluation criteria. Via single factor experiments, relatively better conditions were identified. Mass ratio of the ingredients is: m(soybean lecithin):m(vitamin E):m(cholesterol):m(polyvinyl alcohol)=6:3:3:2. Volume ratio of solvents is: v(chloroform):v(water)=1:3. Ultrasonic treatment time is 2 min. At the optimal conditions, the extent of encapsulation achieves 86.8% with particle size of 62.9 nm. Zeta potential is-10 mv. And the shape of the liposome product is round balls. Suitable temperature for storing the liposome product is recommended as 4 oC. The mass fraction of vitamin E is 8.8% in system.The anhydrous ethanol was used as solvent, preparation of liposome for encapsulating vitamin E. Extent of encapsulation was taken as evaluation criteria. Via single factor experiments and orthogonal test, optimal conditions were identified. The influence factor for preparation of liposome: tween80>vitamin E>ultrasonic treatment>anhydrous ethanol>span80. Mass ratio of the ingredients is: m(egg yolk lecithin):m(vitamin E):m(tween80):m(span80)=14:7:4:4. Volume ratio of solvents is: v(anhydrous ethanol):v(phosphate buffer solution)=1:2.5. Ultrasonic treatment time is 2 min. At the optimal conditions, the extent of encapsulation achieves 85.0% with particle size of 80.0 nm. Zeta potential is-8.0 mv. And the shape of the liposome product is round balls. Suitable temperature for storing the liposome product is recommended as 4 oC. The mass fraction of VE is 7.8% in system.The anhydrous ethanol was used as solvent. Preparation of liposome for encapsulating vitamin E acetate. As well as the liposome for encapsulating coenzyme Q10 were prepared by the same method. Extent of encapsulation was taken as evaluation criteria. Via orthogonal test, optimal conditions were identified about vitamin E acetate liposome. The influence factor for preparation of liposome: tween80>vitamin E acetate>ultrasonic treatment>anhydrous ethanol>span80. Mass ratio of the ingredients is: m(egg yolk lecithin):m(vitamin E acetate):m(tween80):m(span80)=14:7:4:4. Volume ratio of solvents is: v(anhydrous ethanol):v(phosphate buffer solution)=1:2.5. Ultrasonic treatment time is 2 min. At the opitimal conditions, ten extent of encapsulation achieves 82.0% with particle size of 85.7 nm. Zeta potential is-7.0 mv. And the shape of the liposome product is round balls. Suitable temperature for storing the liposome product is recommended as 4 oC. The mass fraction of vitamin E acetate is 7.8% in system. Via orthogonal test, optimal conditions were identified about coenzyme Q10 liposome. The influence factor for preparation of liposome: tween80>coenzyme Q10>ultrasonic treatment>anhydrous ethanol>span80. Mass ratio of the ingredients is: m(egg yolk lecithin):m(coenzyme Q10):m(tween80):m(span80)=14:7:4:4. Volume ratio of solvents is: v(anhydrous ethanol):v(phosphate buffer solution)=1:2.5. Ultrasonic treatment time is 2 min. At the optimal conditions, the extent of encapsulation achieves 84.8% with particle size of 100.0 nm. The zeta potential is-13.0 mv. And the shape of the liposome product is round balls. Suitable temperature for storing the liposome product is recommended as 4 oC. The mass fraction of coenzyme Q10 is 4.0% in system.Preparation of the cosmetics about the moisturing cream and moisturizing lotion, and respectively adding vitamin E liposome 、vitamin E acetate liposome and coenzyme Q10 liposme, in addition to examine the stability of various moisturing cream and moisturizing lotion. The conclusion: the stability of good heat resistance and cold resistantce, the good centrifugal stability. The moisturizer has fine luster. The moisturizing lotion is milk-white. Applying in skin, it make skin well and absorb easily, it is very good at moisturing. So the formula is suitable.