Research On Microbial Molecular Ecology Of Daqing Oilfield Reservoir

Microbial enhanced oil recovery(MEOR) is a third oil recovery method in the world used in oil production, now it has become an important and rapid development of production technology,it uses the beneficial microbial activities by itself or its metabolites to enhance oil recovery. At present, people can use the way of pure culture,some microorganisms in oilfield were isolated and cultured,and have been isolated from the major reservoir with oil flooding some flooding effect strain. However,due to the nature of microbial species can develop only rarely in the possession and total number of bacteria in part,which makes the traditional isolation technology can only reflect the characteristics of a small amount of bacteria detected by microbial reservoirs.In recent years,with the rapid development of microbial molecular ecology,especially the 16 S r DNA clone library, real-time fluorescence in situ hybridization,based on DGGE(denaturing gradient gel electrophoresis) technology improvement and development,microbial molecular ecology technology has been widely used to study the community structure and population dynamics of microorganisms in reservoir.Analysis of microbial community structure and function,and the changes in community has been studied.To make people more comprehensive,objective,accurate and timely understanding of the reservoir microbial community structure and population dynamics,can reveal the diversity of oil reservoir microorganism at the molecular level,to promote understanding of the diversity of the reservoir microorganisms,and deeply affected the microbial recovery and application direction.In this study,using the method of gene clone library analysis of Daqing oilfield after polymer flooding reservoir(three production plants and four production plants),low permeability reservoir(ten plant oil) produced in typical block structure characteristics of liquid bacteria.These blocks were constructed by bacterial and archaeal 16 Sr DNA gene clone library,and MEGA5.1 sequence analysis and phylogenetic analysis using NCBI database.The results show that,the three production plant belongs to the genus of Uncultured bacterium bacteria clone,total bacteria 82%.The advantage of Archaea belongs to the genus from high spring environment,has strong adaptability to the underground high temperature environment Crenarchaeotal sp.,the total bacteria 36%. Four production plant bacteria bacteria from the Uncultured bacterium,accounting for 60.5% of total bacteria.The advantage of Archaea belongs to the genus of Uncultured bacterium,accounted for more than 34% of the bacteria,followed by alkaline soil from the environment Rhizobium sp.reached 29.75% of the total number of bacteria.The ten production plant belongs to the genus of bacteria with degradation of acetonitrile,degradation characteristics of chlorophenol Acinetobacter sp. accounted for 43.5%.The advantage of archaea,bacteria were derived from alkaline soil environment,with hydrogen production and cellulase effect of Pantoea sp. to total bacteria 57.75%.This paper uses the 16 Sr DNA gene clone library analysis reservoir microbial community composition and dominant species,the main production function analysis for bacteria,microbial enhanced oil recovery(MEOR) provides guidance for flora selection,At the same time,the application of Microbial Molecular Ecology Techniques for determining reservoir microbial enhanced oil recovery technology feasibility,compatibility,development and application of strain and further guidance in the process of microbial enhanced oil recovery.Then the optimization provides a reliable basis for the technology of EOR of microorganism.