Microbial Diversity Research On Yellow Catfish(Pseudobagrus Fulvidraco) Gastrointestinal And Culture Environment Of Ipomoea Aquatica Floating-Bed Used In Fish Pond

Yellow catfish(Pseudobagrus fulvidraco) is an important category of China’s special aquaculture, through large-scale cultivation experiment, the removal capacity of nitrogen and phosphorus by Ipomoea aquatica Forsk planted on artificial floating beds were emphatically studied, Illumina high-throughput sequencing technology was used to analysis microbial diversity on yellow catfish(Pseudobagrus fulvidraco) gastrointestinal and culture environment of Ipomoea Aquatica floating-bed used in fish pond, which lays the theoretical foundation for the further promotion of Ipomoea aquatica Forsk planted on artificial floating beds ponds culture mode and science and health culture of yellow catfish. The main results of this study are as follows:2014 June- October, we laying Ipomoea aquatica floating bed in an area of 8000 square meters of yellow catfish ponds, and coverage was 7%, therwise no floating bed of the pond as the control, through the analysis of nitrogen and phosphorus in water show that, the Ipomoea aquatica floating bed show purification effect on the pond water play.This study collected 13 samles from yellow catfish intestine pond water, sediment, roots of Ipomoea aquatica, feed of experimental group and control group, high-throughput sequencing technology was used to sequence V3-V4 variable region of 16 S r RNA gene. After sequencing found that all samples have high bacterial diversity, beteween 9489 and 300,60 effective sequences were read from each sample, resulting in a total of 204,575 sequences for 13 samples, these sequences were clustered into 15220 OTUs at the 97% similarity level. 123-164 OTUs were detected in intestinal tract samples, 879-1660 OTUs were detect in water samples, 1568-2098 OTUs were detected in sediment samples, 1121 OTUs were detect in the samples of Ipomoea aquatica root, 214 OTUs in the detection of pellet feed. The microbial diversity is different from different breeding period of yellow catfish. Proteobacteria were dominant bacteria in yellow catfish at early periods, the relative abundance was 88.56%, significantly higher than the end periods of the experimental group and the control group(14.56% and 36.72%); Fusobacteria was significantly increased in the end periods, the relative abundance was 4.07% at early periods, but at end periods the relative abundance was 32.52% and 17.48% in experimental groups and control groups. The results also show that between different culture environment the microbial diversity of yellow catfish were different, the diversity of experimental group is lower than the control group. More than 99% of the sequences in the pellet feed consists of three components: Cyanobacteria、Proteobacteria、Firmicutes. The dominant bacteria in water and sediment samples both are Proteobacteria, the relative abundance of 54.40%-59.59% in water samples, and the relative abundance in sediment samples for 30.07%-47.31%. Water samples and Ipomoea aquatica root sample were not significantly different, the surrounding Ipomoea aquatica root can adsorb the ammonia oxidizing bacteria effectively.The principal component analysis, principal co-ordinates analysis and cluster analysis showed build Ipomoea aquatica planted on artificial floating beds can change the microorganisms of water and sediment, but no significant relationship between changes of microbial community in water and sediment and changes of microbial community in intestine of yellow catfish, the bacterial composition of yellow catfish intestinal and pellet feed has certain correlation.Through the above research, the Ipomoea aquatica Forsk planted on artificial floating beds in the production process can cleansing aquaculture water quality effectively, through high-throughput sequencing technology proved the difference yellow catfish intestinal flora in different breeding patterns, the intestinal flora and bacterial distribution of pellets feed have a certain relevance, breeding in the water and sediment bacteria almost had no effect on the intestinal flora. This study provides a theoretical support for the future study on intestinal flora of yellow catfish,and provide a more comprehensive understanding of Ipomoea aquatica Forsk planted on artificial floating beds changes the flora structure of yellow catfish intestinal flora structure and culture environment.