| Diet is the main way for PAHs to enter the human body. PAHs can easily enrich in fatty foods such as aquatic product as for its lipophilic property. Xiamen is located along the southeast coast where aquatic product consumption has a large proportion in residents’ daily diet, therefore the edible security of aquatic product is now deeply concerned. This paper establishes a method using high performance liquid chromatography with fluorescence and ultraviolet detector to detect 16 PAHs in aquatic product, investgating several kinds of aquatic product that have large consumption in daily diet for Xiamen citizen and analizing 16 PAHs in aquatic product and make preliminary dietary exposure assessment, mainly studies are as follows:(1) This study established a method using high performance liquid chromatography with fluorescence and ultraviolet detector to detect 16 PAHs in aquatic product. After being optimized, the method’s linear range was 1~50 μg·kg-1, the linear correlation coefficient was greater than 0.997.When 16 PAHs were added at a concentration of 5.0~20 μg·kg-1, the recoveries were 71.2%~101%. The limit of quantitation of Pyrene, benzo [a] anthracene, Chrysene, benzo [b] fluoranthene, benzo [k] fluoranthene, benzo [a] pyrene and dibenzo [a, h] anthracene was 0.5 μg·kg-1, The limit of quantitation of naphthalene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, benzo [g, h, i] perylene and indeno [1, 2, 3-cd] pyrene was 1.0 μg·kg-1, acenaphthylene quantitation limit of 5.0 μg·kg-1. The intra-assay and inter-assay method relative standard deviations were less than 15%. The method is in accordance with the requirements of the national standard detectionmethods.(2) 198 aquatic product samples were collected from supermarkets and farmers markets in Xiamen, of which a total of 90 fish samples, 30 shrimp samples, 60 mussel samples and 18 crab samples were collected. PAHs of the testing samples were detected using the method of the present experimental study, the results are as follows:1) 16 PAHs in aquatic product sold in markets in Xiamen were at low levels, of which naphthalene, fluorene, phenanthrene, fluoranthene, pyrene and benzo [g, h, i] perylene had high detection rates, the rest 10 PAHs were low. The total PAHs content in mussel was the highest,in which the average total amount was 7.72 μg·kg-1, followed by fish(6.23 μg·kg-1) and shrimp(5.69 μg·kg-1), the lowest was crabs(5.10 μg·kg-1). The content of PAHs was lower in cultured aquatic product than in wild aquatic product.2) Study found that PAHs in aquatic product was closely related to the environment that the sea creatures live and the body fat content and other factors, the higher the fat content, the higher the content of PAHs in the living environment, the higher residue levels of PAHs in aquatic product.3) Low molecular weight PAHs in aquatic product accounted for the highest proportion of PAHs, reaching 72.7%~85.1%. Moderate molecular weight PAHs in total PAHs have a ratio of 5.3% ~16.1%. High molecular weight PAHs have a proportion of 9.6%~16.8% in total PAHs.(3) The intake and cancer risk value of PAHs for the residents was got according to the dietary exposure model and health risk assessment model of USEPA. The results showed that:The intake of PAHs for Xiamen residents was at low levels. Regardless of sex, the intake of PAHs was the highest for 15~59 year-olds, followed by 60 years-olds, the lowest was 0~14 year-olds.The cancer risk for all population in Xiamen were below acceptable levels and the priority risk value. Regardless of sex, the lifetime cancer risk of PAHs was as follows: 0~14-year-olds > 60 years-olds > 15~59 year-olds. The lifetime cancer risk of 0~14 year-olds for men was higher than women. For 15~59 year-olds and 60 years-olds, the lifetime cancer risk of PAHs for women was higher than men. |
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