| The aging population has become a global problem. The dilemma of getting old before getting rich will seriously restrict our country’s sustainable development. Nutritional regulation is an important way to delay senescence. Limiting the intake of macronutrients and supplying additional ingredients except for necessary nutrients, such as phytochemicals which have antioxidant effects are traditional dietary intervention measures for delaying senility are. However, recent studies have indicated that there are limitations in dietary restriction.The wild Canton-S Drosophila melanogaster was used as the model object. Effects of 9 different proportions of sucrose (S) and yeast (P)(S 2%P 2%, S 2%P 4%, S 2%P 8%, S 2% P 16%, S 4%P 2%, S 4%P 4%, S 8%P 2%, S 8% P 8% and S 16% P 2%) on lifespan of Drosophila were observed. Effects of curcumin intervention to the 9 diets were analyzed. Effects of different diets and curcumin intervention on food consumption and fecundity in Drosophila were also studied.1. Effects of dietary sugar and protein proportion on lifespan in DrosophilaThe result shows that the change of the S/P ratio will significantly affect the lifespan of Drosophila fed with different sucrose and protein proportion. Lifespan of Drosophila was the longest as 30.12 days when the S/P ratio was 4%/2%(C5), relatively longer when the ratio was 2%/2%(C1) and 2%/4%(C2), however, the shortest as 17.39days when the ratio was 2%/16%. There’s a huge difference between the function of protein and sugar. Lifespan was longer with 2% sugar and 2%-4% protein, while it was shortened when protein was overdose, for instance, lifespan was shortened by 42.46% when the ratio changed from 2%/4% to 2%/16%. On the contrary, overdose of sugar (S/P=16%/2%) shortened the lifespan by 8.79% while the lowered range is 33.47% less than the ratio of 2%/16%. The results indicate that proper sugar and protein proportion help longer life and the proportion imbalance will shorten lifespan. Protein overdose helped greater to aging compared with sugar overdose.2. The effects of curcumin intervention on aging induced by dietary sugar and protein proportion imbalance in Drosophila.Effects of curcumin intervention was observed and the 9 different diets were made as control. The results indicate that there are obvious interaction between curcumin and macronutritients, and the supplement of curcumin lifelong will improve the shortened lifespan induced by the imbalance of S/P. Comparing with the control group, lifespans of Drosophila fed with the ratio of S/P as 4%/4%,8%/2%,8%/8% and 16%/2% ware extended by 19.16%,30.58%,12.42% and 15.53%, among which changed the most from 23.15 days to 30.17 days when the S/P ratio was 8%/2%(T7). Meanwhile, the effect of sugar overdose was improved better than that of protein overdose, for example, when curcumin was added, the lifespans were extended by(S/P =8%/8%)12.42% and(S/P=16%/2%)15.53% compared with (S/P=2%/16%, extended 4.29%),8%/8%dietary which extended by 8.13% and 16%/2% dietary which extended by 11.14%.The effects of curcumin intervention on different development stages was analyzed. The results show that the effects on early life(within 20 days since emergence) intervention and on lifelong intervention are similar. Compared with the control groups, lifespans of Drosophila fed with the ratio of S/P 4%/4%,8%/2%,8%/8% and 16%/2% was extended by 19.73%,34.90%,20.60% and 16.37%, among which improved the most as the ratio of 8%/2%. The effect was, however, not obvious on lifespan of Drosophila fed with proper proportion of sugar and protein (S/P=4%/2%).Effects of later life (after 20 days since emergence) curcumin intervention on extending lifespan were better when the proportions of sugar and protein were lower. Compared with the control groups, lifespans of Drosophila fed with the ratio of S/P as 2%/2%,2%/4% and 4%/4% were extended by 27.79%,21.01% and 19.86% when curcumin was added, among which changed the most from 28.54 days to 36.19 days when the S/P ratio was 2%/2%(Al).3. Effects of sugar and protein proportion and curcumin intervention on food consumption and fecundity.Food consumption of Drosophila was measured by adding 0.5% Erioglaucine disodium salt into medium. The results showed that dietary sugar/protein ratio and curcumin supplement both affect Drosophila’s food consumption. Compared to the control groups, the presence of curcumin led to a general decline of food consumption, among which it decreased by 77.75%,22.18%,36.69%,43.94% and 39.90% when the S/P ratio was 2%/2%, 2%/8%,4%/2%,8%/2% and 8%/8%.Increasing protein content helped increase egg amount, while the presence of curcumin decreased it. Compared with the control groups, curcumin decreased egg amount by38.18%, 13.59%,41.42% and 55.28% as the S/P ratio 2%/2%,2%/16%,2%4% and 8%/2%. It can be inferred that the anti-aging effect of curcumin intervention is concerned with the involved decreased energy intake and egg amount.In conclusion, proper S/P proportion helps extend lifespan of Drosophila; supplement of curcumin helps improve premature aging in Drosophila induced by improper S/P proportion; effects of intervention on different life stages are concerned with S/P ratio. The anti-aging effect of curcumin intervention is concerned with the involved decreased energy intake and egg amount. The results will provide scientific basis to anti-aging by regulation of dietary and curcumin supplement. |
PDF Full Text Request