Preparation And Properties Characteristics Of Quaternized Chitosan With Iodine

Chitin is the second largest natural renewable resources next to cellulose, and chitosan(CTS), natural alkaline polysaccharide, is the production of the deacelation. So CTS has unique physicochemical characteristics and biological activities, and it has been widely applied to medical materials and functional materials and so on. However, CTS shows some limitations such as poor solubility, which limits its widespread applications. This study is to improve the water solubility of chitosan and increase antibacterial activity at the same time, which can expand the scope of its application.By using the technology of “Amino protection-Modified reaction-Amino deprotection and restoration”, 3-chloro-2-hydroxypropyl three methyl ammonium chloride and ammonium thiocyanate were grafted into the C2 position and C6 position respectively, and then O-HTCC-ATU was prepared. Various factors on the yield of O-HTCC-ATU were discussed, and the preparation programs were optimized and tested with L18(37) orthogonal. O-HTCC-ATU was analyzed by means of FIIR spectrum, 1HNMR spectroscopy and TG. The results indicate that the optimum conditions for the preparation of O-HTCC-ATU were as follows: the quantity of benzaldehyde was10 m L, the reaction time for B-CTS with CTA was 7h, the reaction temperature for B-CTS with CTA was 70℃, the quality for m(CTA) with m(B-CTS) was 1:4, the reaction time for O-HTCC with ATU was 5h, the reaction temperature for O-HTCC with ATU was 70℃, and the quality for m(O-HTCC) with m(ATU) was 1:3. Although different functional groups were introduced, which increased its water-soluble, but the thermal stability of O-HTCC-ATU was weakened greatly.O-HTCC-ATU gelatin films and O-HTCC-ATU-I2 gelatin films were prepared by a solution method. Various factors on the mechanical and moisture absorption properties of O-HTCC-ATU gelatin films were discussed, and the preparation programs were optimized and tested with L9(34) orthogonal. Gelatin films were analyzed by means of FIIR spectrum, XRD, SEM, TG, iodometric titration, iodine releasing and antibacterial activity test. The results indicate that the optimum conditions for the preparation of O-HTCC-ATU gelatin films were as follows: the content of gelatin was 20%, the reaction time was 7h, the reaction temperature was 50℃, and the content of PVP was 20%. The adsorption of iodine onto the gelatin film increased along with the increasing iodine concentration of ethanol. The releasing results of O-HTCC-ATU-I2 gelatin films in the phosphate buffer(p H =7) show that the O-HTCC-ATU-I2 gelatin films release iodine showly and get balance after 60 minutes. The molecular structure of O-HTCC-ATU-I2 gelatin films was changed slightly because of the addition of iodine, and each component had good compatibility at the same time. The antimicrobial test results show that the antibacterial diameters of O-HTCC-ATU-I2 gelatin films(ω(I2) =21.76%) against E. coli and S. aureus were(42±1)mm and(40±1)mm, respectively, indicating its significant bacteriostasis degree.O-HTCC-ATU/PVA blend films and O-HTCC-ATU/PVA-I2 blend films were prepared by Chemical cross linking method. Various factors on the mechanical and moisture absorption properties of O-HTCC-ATU/PVA blend films were discussed, and the preparation programs were optimized and tested with L9(34) orthogonal. Blend films were analyzed by means of FIIR spectrum, XRD, SEM, TG, iodometric titration, iodine releasing and antibacterial activity test. The results indicate that the optimum conditions for the preparation of O-HTCC-ATU/PVA blend films were as follows: the quality for m(PVA) with m(O-HTCC-ATU) was 30/70, the content of glutaraldehyde was 2%, the reaction time was 1.5h, and the reaction temperature was 70℃. The adsorption of iodine onto the blend films increased along with the increasing iodine concentration of ethanol. The releasing results of O-HTCC-ATU/PVA-I2 blend films in the phosphate buffer(p H =7) show that the O-HTCC-ATU/PVA-I2 blend films release iodine quickly in the first 20 minutes and get balance after 65 minutes. The antimicrobial test results show that the antibacterial diameters of O-HTCC-ATU/PVA-I2 blend films(ω(I2)=15.36%) against E. coli and S. aureus were(42±1)mm and(40±1)mm, respectively, indicating its significant bacteriostasis degree.