| High performance liquid chromatography-resonance Rayleigh scattering (HPLC-RRS) method integrates the high sensitivity of RRS and the high selectivity of HPLC. As HPLC-RRS method was established, the problems of low selectivity and the poor ability of simultaneous determination for multicomponents were better solved, and the applications of Resonance Rayleigh scattering was further expanded as well. The outstanding advantages of high performance liquid chromatography-Resonance Rayleigh scattering is that the chromatographic signal generation does not depend on the chemical properties of the analyte itself, So it is particularly of great advantage to detect the substance which without UV absorption and fluorescent.This paper mainly studies the application of HPLC-RRS combined fluoroquinolone antibiotics and the body of dopamine and determination of vitamin C by technology, and the application of RRS method for the determination of vitamin C. Determination of several kinds of drugs, experimental obtained good results. The experiment explored the optimum reaction conditions and influencing factors, and the reaction mechanism is discussed andverified. This paper consists of the introduction and the main text is composed of two parts,including the introduction part briefly introduces method and Resonance Rayleigh scattering method of high performance liquid chromatography, and introduces the basicprinciple, application status and application prospect of HPLC-RRS coupling method.Research Report part mainly the following three parts.l.High performance liquid chromatography coupled with resonance Rayleighscattering for the detection of three fluoroquinolones in urineA reliable and versatile high performance liquid chromatography coupled with resonance Rayleigh scattering method was established for the determination of three fluoroquinolones, including levofloxacin, norfloxacin and enrofloxacin in water sample and human urine sample. In pH 4.4-4.6 Britton-Robinson buffer medium, the fluoroquinolones separated by high performance liquid chromatography could react with erythrosine to form 1:1 ion-association complexes, which could make contributions to the great enhancement of RRS. The resonance Rayleigh scattering signal was recorded at λex=λem-330 nm. The resonance Rayleigh scattering spectral characteristics of the drugs and the experimental conditions such as pH, detection wavelength, erythrosine concentration, flow rate, the length of reaction tube were studied. Quantum chemistry calculation, Fourier transform infrared spectroscopy and absorption spectroscopy were used to discuss the reaction mechanism. The recoveries of samples added standard ranged from 97.53% to 102.00%, and the relative standard deviation was below 4.64%. The limit of detection (S/N=3) of 0.12-0.28μg mL-1 was reached, and the linear regression coefficients were all above 0.999. The proposed method was proved as a simple, low cost and high sensitivity method.2. A highly sensitive resonance Rayleigh scattering method for the determination of Vitamin C based on the formation of Zirconium hexacyanoferrate (II) nanoparticlesIn pH 2.55 HC1 solution, Vitamin C (VC) could deoxidize [Fe(CN)6]3-producing [Fe(CN)6]4-, which reacted with Zr (IV) to form Zr[Fe(CN)$] complex, then aggregated to produce{Zr[Fe(CN)6]}n nanoparticles by virtue of hydrophobic force and Van der Waals force. The intensity of RRS at 340 nm is directly proportional to the concentration of VC in the range of 0.1-1.45 μgmL-1, and the detection limit (3s/k) is 0.128 ng-mL-1. In this work, the spectral characteristics of RRS, the optimum condition, the reaction mechanism of the reaction, and their influencing factors especially the effect of organic solvent have been studied. The sensitive, rapid and simple method based on resonance Rayleigh scattering was successfully applied to determine trace amount VC in VC injection and tablet.3. High performance liquid chromatography coupled with resonance Rayleigh scattering for the detection of Vitamin C and dopamineIn the pH 2.55 hydrochloric acid, vitamin C or dopamine could form (Zr[Fe(CN)6]}n nanoparticles with [Fe(CN)6]3- and Zr (IV) after a series of reaction, which makes the resonance Rayleigh scattering signal at 340 nm significantly enhanced. Based on this, a good selectivity and efficient method was established for the determination of vitamin C and dopamine in human urine, by high performance liquid chromatography coupled with Resonance Rayleigh scattering. This paper studied the effects of pH, probe concentration, flow rate on the reaction system, and ultraviolet absorption spectroscopy and scanning electron microscope were used to discuss the reaction mechanism. |
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