The Design, Synthesis And Application Of Fluorescent Probes Based On Naphtha Anhydride

Fluorescent probe have been wildly used in the field of biological, clinical, environmental and chemistry for its simplicity, good selectivity, high sensitivity, low detection limit, and temporal and spatial detection.Here, we described the design and synthesize of three naphthalimide-based fluorescent probes and its potential applications. The main contents of this thesis are:（1）We have designed and synthesized a naphthalimide-based fluorescent probe, ZS1, for the detection of zinc ions in aqueous solution. ZS1 displays an excellent fluorescent selectivity for Zn₂₊ with an enhanced red-shift in both absorption and emission resulting from the Zn₂₊-triggered deprotonation of amide group. ZS1 could detect as low as 7.2 n M Zn₂₊. More importantly, it displayed specific and sensitive recognition to Zn₂₊ and specially avoided the interference of Cd₂₊ in aqueous solution. Moreover, probe ZS1 could be successfully utilized in living cells for real-time detection of Zn₂₊.（2）We have designed and synthesized a naphthalimide-based fluorescent probe, NAP2, for the detection of copper ions in aqueous solution. NAP2 displays an excellent fluorescent selectivity for Cu₂₊ with an obvious quenching of fluorescence emission resulting from the paramagnetism of Cu₂₊. NAP2 could detect as low as 9.9 n M Cu₂₊. In a word, it displayed specific and sensitive recognition to Cu₂₊ in aqueous solution.（3）We have designed and synthesized a new type of naphthalimide-based fluorescent probe, NAP3, for the detection of mercury ions in aqueous solution. NAP3 displays an excellent fluorescent selectivity for Hg₂₊ with a significant enhanced intensity of fluorescence emission resulting from the Photoinduced Electron Transfer（PET）. In the presence of Hg₂₊, which blocks PET effect of 4-piperazine-1,8-naphthalimide, has shows about 20-fold fluorescence increase and exhibits high sensitivity for the response to Hg₂₊.