Researches For Influence Of Phosphatase Activity On Transformation Of Organic Phosphorus In Wetland Soil

Wetland plants rhizosphere soil exist a large number of phosphatases, which can catalyze the hydrolysis of phosphoric Acid Esters or phosphoric anhydride and its activity directly affects the transformation of soil organic phosphorus transformation and bio-availability. Soil phosphatase activity is used to characterize the soil fertility status to a certain extent, especially the status of phosphorus. The Yeyahu Wetland Nature Reserve is the largest area in Beijing, it was selected as the object in this study. Variations of phosphomonoesterase activity, phosphodiesterase activity and phytase activity with plants growth were studied. Meanwhile, wetlands soil incubation was carried out to study the influences of organic phosphorus fractions and quantity in different periods after application of outside source of phytase.In 2013, three kinds of plant non-rhizosphere soil phosphomonoesterase, phosphodiesterase and phytase activity were in the range of 0.21-0.38 (mgPhOH·g-1·h-1),24.5-83.20 (μgPN·g-1·h-1) and 1.24-4.03 (μgP·g-1·min-1) respectively. Plant rhizosphere soil phosphomonoesterase, phosphodiesterase and phytase activity was in the range of 1.75-3.78 (mgPhOH·g-1·Kh-1),112.1-306.54 (μgPN·g-1·h-1) and 6.82-13.64 (μgP·g-1·min-1) respectively. In 2014, three kinds of plant non-rhizosphere soil phosphomonoesterase, phosphodiesterase and phytase activity was in the range of 0.12-0.82 (mgPhOH·g-1·h-1),40.7-103.2 (μgPN·g-1·h-1) and 9.61-19.22 (ugP·g-1·min-1) respectively. Plant rhizosphere soil phosphomonoesterase, phosphodiesterase and phytase activity was in the range of 1.34-3.47 (mgPhOH@g-1·h-1),113.1-170.4 (μgPN·g-1·h-1) and 19.53-29.14 (μgPN·g-1·min-1) respectively. Results showed that plant rhizosphere soil phosphatase activity was higher than plant non-rhizosphere soil.There was a significant positive correlation between labile organic phosphorus and phosphatases. There was a significant negative correlation between highly resistant organic phosphorus and phytase, so there were obvious effects in the process of degrade high stability compounds in soil by phytase. The effectiveness of organic P can be improved by phytases under the appropriate conditions for short periods. The outside source of phytase increased the contents of labile, moderately labile organic P in soil which is higher than CK. During the cultivation periods, the contents of labile organic P increased by 173.05%,158.16%, and 122.75%, respectively, and the contents of moderately labile organic P increased by 84.22%,68.7% and 53.92%, respectively, after each treatment. In addition, the contents of moderately resistant and highly resistant organic P when added phytases were lower than that of CK. The contents of soil moderately resistant organic P decreased by 62.81%,37.31% and 26.14%, respectively, and the contents of highly resistant organic P decreased by 94.71%,87.66%and 79.52%, respectively.