Isolation, Characterization And Function Studies Of Super Fullerenes Embedded Gd³⁺ And Its Water Soluble Derivative

In this paper, super fullerenes embedded Gadolinium(Ⅲ) which were purified and its derivatives fullerols were characterized and further related functions researching. Super fullerenes were characterized by TEM, TEM-EDS, elemental analysis, FSEM, FT-IR, Raman spectra, and its absorption properties were studied. Meanwhile the super fullerols embedded Gadolinium(Ⅲ) were performed and separated, that were characterized and further functions studied by AFM, NMR, XPS, FT-IR, fluorescence spectra and UV-VIS.Results showed that each six-membered ring was surrounded by six six-member rings, and the tremendous polyhedral cage structure was formed by the alternating six-membered ring structure. A small number of double deck super fullerenes embedded Gadolinium(Ⅲ) were found. The chemical shift value of 13 C NMR and 1H NMR spectroscopy were ? 168.54 and 3.44 respectively, which proved that although the super fullerenes embedded Gadolinium(Ⅲ) were completely alcoholized, the structure of molecule skeleton remained. The electronic force binding on the tracks was changing, therefore the self-absorption phenomenon was found in the fluorescence spectrum. FT-IR showed that super fullerenes embedded Gadolinium(Ⅲ) and their fullerols were combining with ferrocene with chemical bonds in the reaction of solid state materials. The largest Reflection Loss(RL) value of super fullerenes increased with the increase of the specimen thickness. Under the condition of turning the electromagnetic energy into heat energy, which led to the changing of electromagnetic parameters. The electromagnetic wave transmission loss mainly transformed from electric loss way to the magnetic loss within a certain broad frequency range.In physiological environment, the interaction of ct-DNA with super fullerols embedded Gadolinium(Ⅲ) were studied by means of spectral methods, cyclic voltammetric curves, viscosity experiment, Scatchard method and thermodynamic analysis. And the interplay between BSA and fullerols was researched with spectral methods. The thermodynamic parameters were calculated as follows: ΔrSm? =8.38 fluorescence strength of super fullerols with base: G: 18.36%; C: 19.20%; A: 19.56%; T: 17.15%. All the experimental testimony showed that the binding modality between super fullerols embedded Gadolinium(Ⅲ) and ct-DNA were electrostatic bonding and the outer groove binding. The interaction process between super fullerols and BSA was controlled by enthalpy with dynamic quenching. It proved that the interaction mode between them did involve van der Waals and hydrogen bond. Free radical scavenging experiment revealed that super fullerols embedded Gadolinium(Ⅲ) had a high efficiency if used to scavenge ?OH free radical only.